Automated Glycan Assembly of Plant Cell Wall Oligosaccharides.

Fabian Pfrengle

Journal: Methods in molecular biology (Clifton, N.J.) 2021;2149():503-512

PMID: 32617953

Abstract

Synthetic cell wall oligosaccharides are promising molecular tools for investigating the structure and function of plant cell walls. Their well-defined structure and high purity prevents misinterpretations of experimental data, and the possibility to introduce chemical handles provides means for easier localization and detection. Automated glycan assembly as emerged has a powerful new method for the efficient preparation of oligosaccharide libraries. We recently made use of this technology to prepare a collection of plant cell wall glycans for cell wall research. In this chapter, detailed experimental procedures for the automated synthesis of oligosaccharides that are ready for use in biological assays are described.

Address: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany. [email protected].

DOI: 10.1007/978-1-0716-0621-6_28

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https://doi.org/10.1007/978-1-0716-0621-6_28

MeSH Terms: Automation, Laboratory, Cell Wall, Chromatography, High Pressure Liquid, Galactans, Glucans, Glycosylation, Oligosaccharides, Photolysis, Plant Cells, Polysaccharides, Solid-Phase Synthesis Techniques, Xylans