The Antibody Society's antibody validation webinar series.

Jan L A Voskuil, Anita Bandrowski, C Glenn Begley, Andrew R M Bradbury, Andrew D Chalmers, Aldrin V Gomes, Travis Hardcastle, Fridtjof Lund-Johansen, Andreas Plückthun, Giovanna Roncador, Alejandra Solache, Michael J Taussig, James S Trimmer, Cecilia Williams, Simon L Goodman

Journal: mAbs 2021;12(1):1794421

PMID: 32748696

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Abstract

In the wake of the reproducibility crisis and numerous discussions on how commercially available antibodies as research tool contribute to it, The Antibody Society developed a series of 10 webinars to address the issues involved. The webinars were delivered by speakers with both academic and commercial backgrounds. This report highlights the problems, and offers solutions to help the scientific community appropriately identify the right antibodies and to validate them for their research and development projects. Despite the various solutions proposed here, they must be applied on a case-by-case basis. Each antibody must be verified based on the content of the product sheet, and subsequently through experimentation to confirm integrity, specificity and selectivity. Verification needs to focus on the precise application and tissue/cell type for which the antibody will be used, and all verification data must be reported openly. The various approaches discussed here all have caveats, so a combination of solutions must be considered.

Address: Aeonian Biotech Ltd , Marcham, UK.; Center for Research in Biological Systems, University of California , La Jolla, CA, USA.; BioCurate , Parkville, Australia.; Specifica Inc ., Santa Fe, NM, USA.; Department of Biology and Biochemistry, University of Bath , Bath, UK.; CiteAb , Bath, UK.; Department of Neurobiology, Physiology, and Behavior, University of California Davis , Davis, CA, USA.; Horizon Discovery , Waterbeach, Cambridge, UK.; Department of Immunology, Oslo University Hospital , Oslo, Norway.; Department. of Biochemistry, University of Zurich , Zurich, Switzerland.; Monoclonal Antibody Unit, Spanish National Cancer Research Center , Madrid, Spain.; Abcam Plc, Discovery Drive, Cambridge Biomedical Campus , Cambridge, UK.; Cambridge Protein Arrays Ltd., Babraham Research Campus , Cambridge, UK.; Department of Physiology and Membrane Biology, University of California Davis School of Medicine , Davis, CA, USA.; Science for Life Laboratory, Department of Protein Science, KTH Royal Institute of Technology , Solna, Sweden.; Department of Biosciences and Nutrition, Karolinska Institutet , Huddinge, Sweden.; The Antibody Society, Inc ., Framingham, MA, USA.

DOI: 10.1080/19420862.2020.1794421

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MeSH Terms: Antibodies, Monoclonal, Antibody Specificity, Humans, Protein Engineering, Validation Studies as Topic