Nabanita Naskar, Anindita Seal, Senjuti Sinharoy, Sucheta Tripathy, Alexander J Valentine, Aleysia Kleinert, Manidipa Banerjee, Mousumi Poddar Sarkar, Sudipta Tripathi, Upal Das Ghosh, Susanta Lahiri, Karnelia Paul, Gairik Mukherjee, Dipayan Bose, Mohit Kumar, Souvik Mitra, Diya Sen, Haraprasad Naiya, Drishti Mandal, Mayurakshi Nag, Chinmay Saha
Journal: The Plant cell 2020;32(2):486-507
PMID: 31757927
Nitrogen (N) limits crop yield, and improvement of N nutrition remains a key goal for crop research; one approach to improve N nutrition is identifying plant-interacting, N-fixing microbes. JGTA-S1 is a basidiomycetous yeast endophyte of narrowleaf cattail (). JGTA-S1 could not convert nitrate or nitrite to ammonium but harbors diazotrophic (N-fixing) endobacteria () that allow JGTA-S1 to fix N and grow in a N-free environment; moreover, dinitrogen reductase was transcribed in JGTA-S1 even under adequate N. Endobacteria-deficient JGTA-S1 had reduced fitness, which was restored by reintroducing JGTA-S1 colonizes rice (), significantly improving its growth, N content, and relative N-use efficiency. Endofungal plays a significant role in increasing the biomass and ammonium content of rice treated with JGTA-S1; also, JGTA-S1 has better N-fixing ability than free-living and provides fixed N to the plant. Genes involved in N metabolism, N transporters, and -like transcription factors were upregulated in rice roots within 24 h of JGTA-S1 treatment. In association with rice, JGTA-S1 has a filamentous phase and only penetrated filamentous JGTA-S1. Together, these results demonstrate an interkingdom interaction that improves rice N nutrition.
© 2020 American Society of Plant Biologists. All rights reserved.
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