A Tripartite Interaction among the Basidiomycete , N-Fixing Endobacteria, and Rice Improves Plant Nitrogen Nutrition.

Nabanita Naskar, Anindita Seal, Senjuti Sinharoy, Sucheta Tripathy, Alexander J Valentine, Aleysia Kleinert, Manidipa Banerjee, Mousumi Poddar Sarkar, Sudipta Tripathi, Upal Das Ghosh, Susanta Lahiri, Karnelia Paul, Gairik Mukherjee, Dipayan Bose, Mohit Kumar, Souvik Mitra, Diya Sen, Haraprasad Naiya, Drishti Mandal, Mayurakshi Nag, Chinmay Saha

Journal: The Plant cell 2020;32(2):486-507

PMID: 31757927

Abstract

Nitrogen (N) limits crop yield, and improvement of N nutrition remains a key goal for crop research; one approach to improve N nutrition is identifying plant-interacting, N-fixing microbes. JGTA-S1 is a basidiomycetous yeast endophyte of narrowleaf cattail (). JGTA-S1 could not convert nitrate or nitrite to ammonium but harbors diazotrophic (N-fixing) endobacteria () that allow JGTA-S1 to fix N and grow in a N-free environment; moreover, dinitrogen reductase was transcribed in JGTA-S1 even under adequate N. Endobacteria-deficient JGTA-S1 had reduced fitness, which was restored by reintroducing JGTA-S1 colonizes rice (), significantly improving its growth, N content, and relative N-use efficiency. Endofungal plays a significant role in increasing the biomass and ammonium content of rice treated with JGTA-S1; also, JGTA-S1 has better N-fixing ability than free-living and provides fixed N to the plant. Genes involved in N metabolism, N transporters, and -like transcription factors were upregulated in rice roots within 24 h of JGTA-S1 treatment. In association with rice, JGTA-S1 has a filamentous phase and only penetrated filamentous JGTA-S1. Together, these results demonstrate an interkingdom interaction that improves rice N nutrition.

© 2020 American Society of Plant Biologists. All rights reserved.

Address: Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, Kolkata 700019, India.; Department of Endocrinology & Metabolism, Institute of Post Graduate Medical Education & Research and SSKM Hospital, Kolkata 700020, West Bengal, India.; National Institute of Plant Genome Research, New Delhi 110067, India.; ICAR-Indian Institute of Natural Resins and Gums Namkum, Ranchi 834010, Jharkhand, India.; Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Stockholm, SE 75007, Sweden.; Computational Genomics Laboratory, Structural Biology and Bioinformatics Division, Council of Scientific and Industrial Research-Indian Institute of Chemical Biology, Kolkata 700032, India.; Department of Botany, Darjeeling Government College, Darjeeling 734101, India.; Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi 110016, India.; Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata 700064, India.; Saha Institute of Nuclear Physics, Kolkata 700064, India.; Department of Environmental Science, University of Calcutta, Kolkata 700019, India.; P.G. Department of Botany, Bidhannagar College, Kolkata 700026, India.; Agricultural Experimental Farm, Institute of Agricultural Science, University of Calcutta, Kolkata 700144, India.; Department of Botany, University of Calcutta, Kolkata 700019, India.; Botany & Zoology Department, University of Stellenbosch Private Bag X1 Matieland 7602 South Africa.; Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, Kolkata 700019, India [email protected].

Link outs

Bant logo

© Copyright 2026, Nutrition Evidence

NED wishes to thank the following organisations for their support:

We use cookies to improve your experience and analyze site traffic with Google Analytics. By continuing to use our site, you agree to our use of cookies. Learn more.