Integration of comprehensive data and biotechnological tools for industrial applications of Kluyveromyces marxianus.

Mochamad Nurcholis, Noppon Lertwattanasakul, Nadchanok Rodrussamee, Tomoyuki Kosaka, Masayuki Murata, Mamoru Yamada

Journal: Applied microbiology and biotechnology 2020;104(2):475-488

PMID: 31781815

Abstract

Among the so-called non-conventional yeasts, Kluyveromyces marxianus has extremely potent traits that are suitable for industrial applications. Indeed, it has been used for the production of various enzymes, chemicals, and macromolecules in addition to utilization of cell biomass as nutritional materials, feed and probiotics. The yeast is expected to be an efficient ethanol producer with advantages over Saccharomyces cerevisiae in terms of high growth rate, thermotolerance and a wide sugar assimilation spectrum. Results of comprehensive analyses of its genome and transcriptome may accelerate studies for applications of the yeast and may further increase its potential by combination with recent biotechnological tools including the CRISPR/Cas9 system. We thus review published studies by merging with information obtained from comprehensive data including genomic and transcriptomic data, which would be useful for future applications of K. marxianus.

Address: Graduate School of Medicine, Yamaguchi University, Ube, 755-8505, Japan.; Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang, 65145, Indonesia.; Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.; Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515, Japan.; Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi, 753-8515, Japan.; Research Center for Thermotolerant Microbial Resources, Yamaguchi University, Yamaguchi, 753-8515, Japan.; Graduate School of Medicine, Yamaguchi University, Ube, 755-8505, Japan. [email protected].; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi, 753-8515, Japan. [email protected].; Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi, 753-8515, Japan. [email protected].; Research Center for Thermotolerant Microbial Resources, Yamaguchi University, Yamaguchi, 753-8515, Japan. [email protected].

DOI: 10.1007/s00253-019-10224-3

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https://dx.doi.org/10.1007/s00253-019-10224-3

MeSH Terms: Biotechnology, Gene Expression Profiling, Genomics, Industrial Microbiology, Kluyveromyces, Metabolic Engineering