The Main (Glyco) Phospholipid (MPL) of .

Hans-Joachim Freisleben

Journal: International journal of molecular sciences 2020;20(20):5217

PMID: 31640225

Abstract

The main phospholipid (MPL) of DSM 1728 was isolated, purified and physico-chemically characterized by differential scanning calorimetry (DSC)/differential thermal analysis (DTA) for its thermotropic behavior, alone and in mixtures with other lipids, cholesterol, hydrophobic peptides and pore-forming ionophores. Model membranes from MPL were investigated; black lipid membrane, Langmuir-Blodgett monolayer, and liposomes. Laboratory results were compared to computer simulation. MPL forms stable and resistant liposomes with highly proton-impermeable membrane and mixes at certain degree with common bilayer-forming lipids. Monomeric bacteriorhodopsin and ATP synthase from were co-reconstituted and light-driven ATP synthesis measured. This review reports about almost four decades of research on membrane and its MPL as well as transfer of this research to species recently isolated from Indonesian volcanoes.

Address: Goethe-Universität, Gustav-Embden-Zentrum, Laboratory of Microbiological Chemistry, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany. [email protected].; Universitas Indonesia, Medical Research Unit, Faculty of Medicine, Jalan Salemba Raya 6, Jakarta 10430, Indonesia. [email protected].
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