Time dependent appearance of selected apoptotic markers and usefulness of their detection in vitro.

Emil Rudolf, Miroslav Cervinka

Journal: Acta medica (Hradec Kralove) 2003;45(4):135-44

PMID: 12587780

Abstract

Many experiments have demonstrated that some cell lines are resistant to chemically induced apoptosis in vitro, and that apoptosis itself is far from being a homogenous phenomenon. Here we show that 10 microg/ml etoposide elicited only minor changes in Bowes human melanoma cells (temporary decrease in cell viability and proliferation, transient phospatidylserine externalization and caspase-3 activation), which weren't clearly capable to start apoptotic pathway in the entire treated population. On the other hand, potassium chromate at concentration of 150 microg/ml executed cell death bearing some features of apoptosis (cell blebbing, caspase-3 activation and cytoskeletal changes) but lacking or showing weakly others (DNA fragmentation and phospatidylserine externalization). Our results suggest that in detecting apoptosis several fault-proof detection systems are to be used to avoid misleading results and conclusions in each experimental setting.

Address: Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biology and Genetics, Czech Republic. [email protected]

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Research Materials:

https://antibodies.cancer.gov/detail/CPTC-CASP3-1

MeSH Terms: Actins, Annexin A5, Apoptosis, Biomarkers, Caspase 3, Caspases, Cell Division, Chromates, DNA Fragmentation, DNA, Neoplasm, Etoposide, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Humans, Melanoma, Experimental, Microscopy, Video, Phosphatidylserines, Potassium Compounds, Tumor Cells, Cultured