Calm Before the Storm: A Glimpse into the Secondary Metabolism of , the Etiologic Agent of the Sisal Bole Rot.

Gabriel Quintanilha-Peixoto, Rosimére Oliveira Torres, Isabella Mary Alves Reis, Thiago Alves Santos de Oliveira, Dener Eduardo Bortolini, Elizabeth Amélia Alves Duarte, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Eric Roberto Guimarães Rocha Aguiar, Ana Cristina Fermino Soares, Aristóteles Góes-Neto, Alexsandro Branco

Journal: Toxins 2020;11(11):631

PMID: 31671681

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Abstract

is a species of the Nigri section of the genus . In nature, it is usually a saprotroph, decomposing plant material. However, it causes the bole rot disease of (sisal), a plant species used for the extraction of hard natural fibers, causing great economic loss to this culture. In this study, we isolated and sequenced one genome of (isolate CCMB 674 (Collection of Cultures of Microorganisms of Bahia)) from the stem tissues of sisal and performed in silico and wet lab experimental strategies to describe its ability to produce mycotoxins. CCMB 674 possesses 64 secondary metabolite gene clusters (SMGCs) and, under normal conditions, it produces secondary metabolism compounds that could disturb the cellular cycle of sisal or induce abnormalities in plant growth, such as malformin C. This isolate also produces a pigment that might explain the characteristic red color of the affected tissues. Additionally, this isolate is defective for the production of fumonisin B1, and, despite bearing the full cluster for the synthesis of this compound, it did not produce ochratoxin A. Altogether, these results provide new information on possible strategies used by the fungi during the sisal bole rot, helping to better understand this disease and how to control it.

Address: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. [email protected].; Laboratório de Fitoquímica, Universidade Estadual de Feira de Santana, Feira de Santana 44036-900, Bahia, Brazil. [email protected].; Laboratório de Fitoquímica, Universidade Estadual de Feira de Santana, Feira de Santana 44036-900, Bahia, Brazil. [email protected].; Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Feira de Santana 44036-900, Bahia, Brazil. [email protected].; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. [email protected].; Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Cruz das Almas 44380-000, Bahia, Brazil. [email protected].; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. [email protected].; Institute of Veterinary Medicine, University of Göttingen, 37073 Göttingen, Germany. [email protected].; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. [email protected].; Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador 40231-300, Bahia, Brazil. [email protected].; Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Cruz das Almas 44380-000, Bahia, Brazil. [email protected].; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil. [email protected].; Laboratório de Fitoquímica, Universidade Estadual de Feira de Santana, Feira de Santana 44036-900, Bahia, Brazil. [email protected].

DOI: 10.3390/toxins11110631

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MeSH Terms: Agave, Amino Acid Sequence, Aspergillus, Brazil, Cell Cycle, Mycotoxins, Secondary Metabolism