Generation of human induced pluripotent stem cell lines from patients with selective IgA deficiency.

Jonathan Arias-Fuenzalida, Jingwei Yu, Likun Du, Joaquin Custodio, Luigi D Notarangelo, Lennart Hammarström, Qiang Pan-Hammarström

Journal: Stem cell research 2020;41():101613

PMID: 31689593

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Abstract

Selective immunoglobulin-A deficiency (IgAD) is the most common primary immunodeficiency (PID) in the Western world and results in higher susceptibility to infections, autoimmune disorders and malignancies. We generated human induced pluripotent stem cell lines from two patients with selective IgAD, PHAi001 and PHAi002. Patient samples were reprogrammed using non-integrative based methods. Pluripotency of the PHAi001 and PHAi002 cell lines was confirmed by their expression of stem cell markers and capacity to differentiate into cells of the three germ layers. The PHAi001 and PHAi002 lines are a unique resource for experimental modeling of selective IgAD and associated disorders.

Address: Department of Biosciences and Nutrition - NEO, Karolinska Institutet, Sweden. Electronic address: [email protected].; Department of Biosciences and Nutrition - NEO, Karolinska Institutet, Sweden; Department of Lymphoma, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China.; Department of Biosciences and Nutrition - NEO, Karolinska Institutet, Sweden.; Department of Medical Biochemistry and Biophysics, Science for Life Laboratory, Karolinska Institutet, Sweden.; Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, United States.; Department of Laboratory Medicine, Division of Clinical Immunology, Karolinska Institutet, Sweden.; Department of Biosciences and Nutrition - NEO, Karolinska Institutet, Sweden. Electronic address: [email protected].

DOI: 10.1016/j.scr.2019.101613

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MeSH Terms: Adult, Cell Culture Techniques, Cell Line, Female, Humans, IgA Deficiency, Induced Pluripotent Stem Cells