Bioavailability assessment of zinc enriched lactobacillus biomass in a human colon carcinoma cell line (Caco-2).

Vaibhao Kisanrao Lule, Sudhir Kumar Tomar, Prince Chawla, Sarang Pophaly, Suman Kapila, Sumit Arora

Journal: Food chemistry 2020;309():125583

PMID: 31699555

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Abstract

The present study utilizes lactobacilli strains having the potential to accumulate a significant amount of Zinc (Zn) in their biomass and ability to deliver the same mineral in a highly bioavailable form. A human origin Lactobacillus fermentum SR4 and Lactobacillus rhamnosus GG (LGG) were studied for their ability to accumulate Zn by growing them in the medium containing Zn salt. Further, Zn enriched cell lysates were prepared by Ultrasonication, as an organic Zn source. Various functional groups involved in bacterial Zn binding were identified by FT-IR spectroscopy and elemental Zn in bio-chelated cell lysate complex was confirmed by SEM and Energy Dispersive X-ray Spectrometry (EDX). Experimental data demonstrated a significantly higher (P < 0.05) bioavailability of Zn chelated by SR4 followed by LGG i.e., 57% and 48%, as compared to the commercially available inorganic (ZnSo) and even organic (Zinc gluconate) forms tested which has 15.6% and 21.7% respectively.

Address: College of Dairy Technology, Warud (Pusad), MAFSU, Nagpur 445204, India.; National Dairy Research Institute, Karnal, Haryana 132001, India. Electronic address: [email protected].; Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, Punjab 144411, India.; College of Dairy Science and Food Technology, CGKV, Raipur 492006, India.; National Dairy Research Institute, Karnal, Haryana 132001, India.

DOI: 10.1016/j.foodchem.2019.125583

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MeSH Terms: Biological Availability, Caco-2 Cells, Colon, Humans, Limosilactobacillus fermentum, Lacticaseibacillus rhamnosus, Microscopy, Electron, Scanning, Spectroscopy, Fourier Transform Infrared, Zinc