Comparison between polyphenol profile and bioactive response in blackthorn (Prunus spinosa L.) genotypes from north Serbia-from raw data to PCA analysis.

B M Popović, B Blagojević, R Ždero Pavlović, N Mićić, S Bijelić, B Bogdanović, A Mišan, Catarina M M Duarte, Ana Teresa Serra

Journal: Food chemistry 2019;302():125373

PMID: 31442706

Full Text on Elsevier Science View on PubMed

Abstract

The aim of this work was to investigate and compare the phenolic profile of 15 wild growing blackthorn (Prunus spinosa L.) genotypes from the slopes of Fruška Gora mountain in north Serbia. Their effect in inhibiting i) α-amylase and α-glucosidase activities and ii) colorectal cancer cell line (HT29) growth was also studied. Blackthorn fruit extracts exhibited high phenolic content being enrich in anthocyanins. Principal component analysis was used to correlate the bioactive response with phenolic composition. It was found that derivatives quercetin and anthocyanin peonidin are the major contributors of the inhibition of carbohydrates hydrolyzing enzymes as well as with the antiproliferative effect of blackthorn. Among all samples, the genotype from Beška locality showed the higher capacity in inhibiting alpha-amylase, alpha-glucosidase and HT29 cell growth. Because of high anthocyanin content and higher bioactive response, these genotypes could be recommended for the further cultivation and investigation.

Address: Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia. Electronic address: [email protected].; Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.; Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, Serbia.; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal.; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), Oeiras, Portugal; iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, Oeiras, Portugal.

DOI: 10.1016/j.foodchem.2019.125373

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MeSH Terms: Anthocyanins, Antineoplastic Agents, Phytogenic, Antioxidants, Chlorogenic Acid, Chromatography, High Pressure Liquid, Food Analysis, Fruit, Genotype, Glycoside Hydrolase Inhibitors, HT29 Cells, Humans, Plant Extracts, Polyphenols, Principal Component Analysis, Prunus, Quercetin, Quinic Acid, Serbia, alpha-Amylases