A Fixed Combination of Probiotics and Herbal Extracts Attenuates Intestinal Barrier Dysfunction from Inflammatory Stress in an In vitro Model Using Caco-2 Cells.

Veronica Cocetta, Daniela Catanzaro, Vittoria Borgonetti, Eugenio Ragazzi, Maria C Giron, Paolo Governa, Ilaria Carnevali, Marco Biagi, Monica Montopoli

Journal: Recent patents on food, nutrition & agriculture 2019;10(1):62-69

PMID: 30088455

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Abstract

BACKGROUND

Inflammatory Bowel Diseases (IBD), are considered a growing global disease, with about ten million people being affected worldwide. Maintenance of intestinal barrier integrity is crucial for preventing IBD onset and exacerbations. Some recent patents regarding oily formulations containing probiotics (WO2010122107A1 and WO2010103374A9) and the use of probiotics for gastrointestinal complaints (US20110110905A1 and US9057112B2) exist, or are pending application.

OBJECTIVE

In this work, we studied the effect of a fixed combination of registered Lactobacillus reuteri and Lactobacillus acidophilus strains and herbal extracts in an in vitro inflammation experimental model.

METHODS

Caco-2 cell monolayer was exposed to INF-γ+TNF-α or to LPS; Trans Epithelial Electrical Resistance (TEER) and paracellular permeability were investigated. ZO-1 and occludin Tight Junctions (TJs) were also investigated by mean of immunofluorescence.

RESULTS

Pre-treatment with the fixed combination of probiotics and herbal extracts prevented the inflammation-induced TEER decrease, paracellular permeability increase and TJs translocation.

CONCLUSIONS

In summary, the fixed combination of probiotics and herbal extracts investigated in this research was found to be an interesting candidate for targeting the re-establishment of intestinal barrier function in IBD conditions.

Address: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, Italy.; Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, Italy.; Department of Physical Sciences, Earth and Environment, University of Siena, 53100 Siena, Italy.; Department of Physical Sciences, Earth and Environment, University of Siena, 53100 Siena, Italy.; SIFITLab, Italian Society of Phytotherapy, 53100 Siena, Italy.; Schwabe Pharma Italia, 39044 Egna, Bolzano, Italy.; Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 35131 Padova, Italy.; SIFITLab, Italian Society of Phytotherapy, 53100 Siena, Italy.

DOI: 10.2174/2212798410666180808121328

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MeSH Terms: Caco-2 Cells, Humans, Inflammation, Plant Extracts, Probiotics, Stress, Physiological