Adipose-derived stem/progenitor cells from lipoaspirates: A comparison between the Lipivage200-5 liposuction system and the Body-Jet liposuction system.

Sara Taha, Maximilian Michael Saller, Elisabeth Haas, Zsuzsanna Farkas, Attila Aszodi, Riccardo Giunta, Elias Volkmer

Journal: Journal of plastic, reconstructive & aesthetic surgery : JPRAS 2020;73(1):166-175

PMID: 31451404

Full Text on Elsevier Science View on PubMed

Abstract

BACKGROUND

Adipose-derived stem/progenitor cells (ADSPCs) are under investigation in many clinical applications for their regenerative potential in a variety of autoimmune, degenerative, and inflammatory diseases. Adipose tissue, which is mainly harvested by manual liposuction, is the main source of these ADSPCs.

OBJECTIVE

In the past years, a variety of different liposuction devices have been commercialized. To ensure a high quality of obtained ADSPCs, it is crucial to show the advantages and disadvantages of frequently used liposuction methods. For this reason, the objective of this study was to compare ADSPCs harvested by either the suction-assisted LipiVage200-5 or the water-assisted Body-Jet system.

METHODS

The proliferation potential of ADSPCs, harvested from 20 patients, was assessed by cumulative population doublings (cumPD), population doubling time (PDT), colony-forming units (CFU), and cell metabolism assays. To prove the multipotency of the primary isolated cells, ADSPCs were induced to differentiate into adipogenic, osteogenic, and chondrogenic lineages.

RESULTS

Our data show a significantly higher cumPD, as well as a significantly lower PDT for cells obtained by the Body-Jet system. No significant differences were found regarding the CFU efficiency and the cell metabolism. Furthermore, we showed that the adipogenic, osteogenic, and chondrogenic potential of ADSPCs is similar in both groups.

DISCUSSION/CONCLUSION

In our study, we provide evidence that the cell characteristics and the functional properties of ADSPCs isolated after liposuction with different techniques are largely similar. However, we observed a significantly higher cumPD and a slightly higher adipogenic potential in cells isolated after liposuction with the Body-Jet system. Different cannula sizes and sheer stresses in the used methods might play a role here.

Address: Department of Hand, Plastic and Aesthetic Surgery, Ludwig-Maximilians-University (LMU), Pettenkoferstraße. 8a, 80336 Munich, Germany.; Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Ludwig-Maximilians-University (LMU), Nußbaumstraße 20, 80336 Munich, Germany.; Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Ludwig-Maximilians-University (LMU), Nußbaumstraße 20, 80336 Munich, Germany; Department of Hand, Plastic and Aesthetic Surgery, Ludwig-Maximilians-University (LMU), Pettenkoferstraße. 8a, 80336 Munich, Germany.; Experimental Surgery and Regenerative Medicine (ExperiMed), Department of General, Trauma and Reconstructive Surgery, Ludwig-Maximilians-University (LMU), Nußbaumstraße 20, 80336 Munich, Germany; Department of Hand Surgery, Helios Klinikum München West, Steinerweg 5, 8124 Munich, Germany. Electronic address: [email protected].

DOI: 10.1016/j.bjps.2019.06.025

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MeSH Terms: Adipocytes, Adipose Tissue, Adult, Aged, Cell Proliferation, Female, Humans, Lipectomy, Male, Middle Aged, Stem Cell Transplantation, Stem Cells, Tissue Donors, Tissue and Organ Harvesting, Water