Fangling Qin, Lu Yao, Cairui Lu, Cong Li, Yanyan Zhou, Chencan Su, Bang Chen, Yehua Shen
Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2019;129():354-364
PMID: 31071388
The polyphenols profiles of the methanol extracts of bitter apricot [Armeniaca Sibirica (L.)] kernel skins (AKS) were analysed by liquid chromatography-electrospray ionization mass spectrometry. The antioxidan, anticancer effect on HepG2 cell and antibacterial properties of the AKS polyphenol extracts were further characterized in vitro. Polyphenol compounds (35), including nine phenolic acids, thirteen anthocyanins and thirteen flavonoids, were identified in AKS for the first time. The content of apigenin 7-O-glucoside, (cyanidin 3-(4″-acetylrutinoside), 3- (6″-acetylglucoside)-5-glucoside and salicylic acid was relatively high than the others. The AKS polyphenols strongly reduced Fe and exhibited good scavenging activity towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free radicals, 1,1-diphenyl-1-picrylhydrazyl free radicals, hydroxyl radicals, superoxide anions and hydrogen peroxide. The AKS polyphenols could regulate oxidant stress in HepG2 cells by downregulating reduced glutathione, upregulating oxidative glutathione, malondialdehyde and advanced oxidation protein products, and reduced cell viability to induce apoptosis of HepG2 cells in vitro. The AKS polyphenols showed strong antibacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Acetobacter aceti and Bacillus cereus. Therefore, the antioxidant, inhibitory effect on HepG2 cells and antimicrobial activity of the AKS polyphenols were distinct and worthy of further consideration for medical industry applications.
Copyright © 2019. Published by Elsevier Ltd.
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