Brazil nut intake increases circulating miR-454-3p and miR-584-5p in obese women.

Bruna Zavarize Reis, Graziela Biude Silva Duarte, Ernesto Vargas-Mendez, Ludmila Rodrigues Pinto Ferreira, Fernando Barbosa, Cintia Cercato, Marcelo Macedo Rogero, Silvia Maria Franciscato Cozzolino

Journal: Nutrition research (New York, N.Y.) 2020;67():40-52

PMID: 31150916

Full Text on Elsevier Science View on PubMed

Abstract

The Brazil nut is an excellent source of selenium (Se), an essential micronutrient for human health. In this study, we hypothesized that Brazil nut intake modulates circulating microRNAs (miRNAs) in obese women and aimed to evaluate the effects of this nut intake on circulating miRNAs in women with obesity or metabolic syndrome (MetS). A randomized controlled clinical trial was conducted on 54 subjects recruited from the Clinical Hospital in São Paulo, Brazil. Patients were randomly assigned to 2 groups: a Brazil nut group (BN group, n = 29) and a control group (CO group, n = 25); both were monitored for 2 months. BN group members were instructed to consume 1 Brazil nut (approximately 1261 μg/Se) per day; CO group members were instructed not to consume any. Biochemical parameters related to Se status and 25 circulating miRNAs in plasma were evaluated in all patients both at baseline and after 2 months. Expression levels of 2 miRNAs (miR-454-3p and miR-584-5p) were significantly increased after Brazil nut intake. To investigate the effect of MetS on circulating miRNAs at baseline, we performed comparisons between women with MetS (n = 23) and women without MetS (others, n = 31). Circulating miR-375 levels were significantly lower (P = .012) in women with MetS. In conclusion, our findings suggested that a daily intake of 1 Brazil nut increased circulating miR-454-3p and miR-584-5p expression levels in obese women, and our network analysis indicated a link between Se intake, vitamin D metabolism, and calcium homeostasis.

Address: Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil. Electronic address: [email protected].; Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil. Electronic address: [email protected].; Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil. Electronic address: [email protected].; RNA Systems Biology Laboratory (RSBL), Department of Morphology, Institute of Biological Sciences (ICB), Federal University of Minas Gerais (UFMG). Electronic address: [email protected].; Department of Clinical, Toxicological and Bromatological Analysis, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo. Electronic address: [email protected].; Division of Endocrinology and Metabolism, Clinical Hospital, School of Medicine, University of São Paulo, São Paulo, Brazil. Electronic address: [email protected].; Department of Nutrition, School of Public Health, University of São Paulo, São Paulo, Brazil; Food Research Center (FoRC), CEPID-FAPESP, Research Innovation and Dissemination Centers São Paulo Research Foundation, São Paulo 05468-140, Brazil. Electronic address: [email protected].; Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Science, University of São Paulo, São Paulo, Brazil. Electronic address: [email protected].

DOI: 10.1016/j.nutres.2019.05.004

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MeSH Terms: Adult, Bertholletia, Biomarkers, Brazil, Diet, Female, Humans, MicroRNAs, Nuts, Obesity