Shotgun proteomic analysis of quinoa seeds reveals novel lysine-rich seed storage globulins.

Hernán P Burrieza, Axel J Rizzo, Ellen Moura Vale, Vanildo Silveira, Sara Maldonado

Journal: Food chemistry 2019;293():299-306

PMID: 31151615

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Abstract

Quinoa seeds have high protein content and an exceptional balance of amino acids, with higher contents of lysine, methionine and cysteine than common cereals. To date, only three globulins, all of which have a content of lysine mass that does not exceed 3.8%, have been identified in quinoa. To address the protein present in quinoa seeds, TCA/Acetone protein extraction was performed using four different quinoa seed genotypes with contrasting edaphoclimatic origins. Proteins were identified and analyzed using label-free shotgun proteomics followed by in silico analysis, using the three published quinoa genomes. This analysis allowed us to identify sixteen globulins, thirteen of which are novel: nine legumin-like proteins and seven vicilin-like proteins. Seven of the novel proteins contain 7.5% or more of lysine mass, justifying the high content of lysine repeatedly reported in quinoa seeds. No significant differences were found between the four genotypes here analyzed.

Address: Instituto de Biodiversidad y Biología Experimental y Aplicada - Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina; Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.; Instituto de Biodiversidad y Biología Experimental y Aplicada - Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina.; Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil; Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil.; Instituto de Biodiversidad y Biología Experimental y Aplicada - Consejo Nacional de Investigaciones Científicas y Técnicas, Ciudad Autónoma de Buenos Aires, Argentina; Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: [email protected].

DOI: 10.1016/j.foodchem.2019.04.098

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MeSH Terms: Chenopodium quinoa, Genome, Plant, Globulins, Lysine, Phylogeny, Plant Proteins, Proteomics, Seed Storage Proteins, Legumins