How to make the reducing power of H available for in vivo biosyntheses and biotransformations.

Lars Lauterbach, Oliver Lenz

Journal: Current opinion in chemical biology 2019;49():91-96

PMID: 30544016

Full Text on Elsevier Science View on PubMed

Abstract

Solar-driven electrolysis enables sustainable production of molecular hydrogen (H), which represents a cheap and carbon-free reductant. Knallgas bacteria like Ralstonia eutropha are able to split H to supply energy in form of ATP and NADH, which can be subsequently used to power reactions of interest. R. eutropha employs the Calvin-Benson-Bassham cycle for the fixation of CO, which is considered as an abundant and non-competing raw material. In this article, we summarize state-of-the-art approaches for H-driven biosyntheses using engineered R. eutropha. Furthermore, we describe strategies for synthetic H-driven NADH recycling. Major challenges for technical application and future perspectives are discussed.

Address: Technische Universität Berlin, Department of Chemistry, Straße des 17. Juni 135, 10623 Berlin, Germany. Electronic address: [email protected].; Technische Universität Berlin, Department of Chemistry, Straße des 17. Juni 135, 10623 Berlin, Germany.

DOI: 10.1016/j.cbpa.2018.11.020

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MeSH Terms: Biotransformation, Cupriavidus necator, Hydrogen, Metabolic Engineering, NAD, Niacinamide, Oxidation-Reduction