Fungal PQQ-dependent dehydrogenases and their potential in biocatalysis.

Kouta Takeda, Kiwamu Umezawa, Anikó Várnai, Vincent Gh Eijsink, Kiyohiko Igarashi, Makoto Yoshida, Nobuhumi Nakamura

Journal: Current opinion in chemical biology 2019;49():113-121

PMID: 30580186

Full Text on Elsevier Science View on PubMed

Abstract

In 2014, the first fungal pyrroloquinoline-quinone (PQQ)-dependent enzyme was discovered as a pyranose dehydrogenase from the basidiomycete Coprinopsis cinerea (CcPDH). This discovery laid the foundation for a new Auxiliary Activities (AA) family, AA12, in the Carbohydrate-Active enZymes (CAZy) database and revealed a novel enzymatic activity potentially involved in biomass conversion. This review summarizes recent progress made in research on this fungal oxidoreductase and related enzymes. CcPDH consists of the catalytic PQQ-binding AA12 domain, an N-terminal cytochrome b AA8 domain, and a C-terminal family 1 carbohydrate-binding module (CBM1). CcPDH oxidizes 2-keto-d-glucose (d-glucosone), l-fucose, and rare sugars such as d-arabinose and l-galactose, and can activate lytic polysaccharide monooxygenases (LPMOs). Bioinformatic studies suggest a widespread occurrence of quinoproteins in eukaryotes as well as prokaryotes.

Address: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.; Department of Applied Biological Chemistry, Kindai University, Nara 631-8505, Japan.; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), 1432 Ås, Norway.; Department of Biomaterial Sciences, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.; Department of Environmental and Natural Resource Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan. Electronic address: [email protected].

DOI: 10.1016/j.cbpa.2018.12.001

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MeSH Terms: Arabinose, Basidiomycota, Biocatalysis, Fucose, Galactose, Ketoses, Oxidation-Reduction, Oxidoreductases, PQQ Cofactor, Substrate Specificity