Lithium ions (Li) and nanohydroxyapatite (nHAp) doped with Li enhance expression of late osteogenic markers in adipose-derived stem cells. Potential theranostic application of nHAp doped with Li and co-doped with europium (III) and samarium (III) ions.

M Alicka, P Sobierajska, K Kornicka, R J Wiglusz, K Marycz

Journal: Materials science & engineering. C, Materials for biological applications 2019;99():1257-1273

PMID: 30889661

Full Text on Elsevier Science View on PubMed

Abstract

Lithium (Li) ion due to its excellent bioactivity is one of the most well-studied element in bone-tissue engineering. In this study, we fabricated nanohydroxyapatite (nHAp) doped with Li ions (5 mol% Li:nHAp) and co-doped with lanthanide ions. We investigated the effects of nHAp, 5 mol% Li:nHAp or Li alone, on osteogenic differentiation of human Adipose Tissue-derived Stem Cells (hASCs), their proliferation, mitochondrial dynamics and apoptosis. Moreover, we monitored cell proliferation after treatment with samarium (III) (Sm) and europium (III) (Eu) ions co-doped 5 mol% Li:nHAp as well as their luminescent property. The hASCs treated with 5 mol% Li:nHAp and Li ions proliferated more rapidly and differentiated effectively than control cells without undergoing apoptosis. Both, 5 mol% Li:nHAp and Li ions improved osteogenic differentiation of hASCs. Moreover they decreased expression of glycogen synthase kinase 3β (GSK3β) while increased β-catenin mRNA level. In addition, Li, nHAp and 5 mol% Li:nHAp improved mitochondrial dynamics and enhanced expression of neural differentiation marker genes. Collectively, the study indicates on pro-osteogenic and anti-apoptotic properties of nHAp doped with Li and Li alone. Moreover, unique properties of 5 mol% Li:nHAp and 5 mol% Li:nHAp co-doped with rare earth ions, such as Sm and Eu have shed a promising light on their potential application in theranostics.

Address: Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Norwida 27B, Wrocław, Poland.; Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland.; Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Okolna 2, 50-422 Wroclaw, Poland; Centre for Advanced Materials and Smart Structures, Polish Academy of Sciences, Okolna 2, 50-950 Wroclaw, Poland. Electronic address: [email protected].; Department of Experimental Biology, Wroclaw University of Environmental and Life Sciences, Norwida 27B, Wrocław, Poland; Faculty of Veterinary Medicine, Equine Clinic - Equine Surgery, Justus-Liebig-University, 35392 Gießen, Germany. Electronic address: [email protected].

DOI: 10.1016/j.msec.2019.02.073

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MeSH Terms: Apoptosis, Biomarkers, Calcification, Physiologic, Calcium, Cell Differentiation, Cell Proliferation, Cell Survival, Cytoskeleton, Durapatite, Europium, Humans, Ions, Leptin, Lithium, Mesenchymal Stem Cells, MicroRNAs, Mitochondrial Dynamics, Nanoparticles, Nestin, Neurofilament Proteins, Osteogenesis, Osteopontin, RNA, Messenger, Samarium, Spectroscopy, Fourier Transform Infrared, Theranostic Nanomedicine, X-Ray Diffraction