Pseudogene repair driven by selection pressure applied in experimental evolution.

Amitesh Anand, Connor A Olson, Laurence Yang, Anand V Sastry, Edward Catoiu, Kumari Sonal Choudhary, Patrick V Phaneuf, Troy E Sandberg, Sibei Xu, Ying Hefner, Richard Szubin, Adam M Feist, Bernhard O Palsson

Journal: Nature microbiology 2019;4(3):386-389

PMID: 30692668

Full Text on Nature Publishing Group View on PubMed

Abstract

Pseudogenes represent open reading frames that have been damaged by mutations, rendering the gene product non-functional. Pseudogenes are found in many genomes and are not always eliminated, even if they are potentially 'wasteful'. This raises a fundamental question about their prevalence. Here we report pseudogene efeU repair that restores the iron uptake system of Escherichia coli under a designed selection pressure during adaptive laboratory evolution.

Address: Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark.; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA. [email protected].; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark. [email protected].

DOI: 10.1038/s41564-018-0340-2

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MeSH Terms: Cation Transport Proteins, DNA Mismatch Repair, Directed Molecular Evolution, Escherichia coli, Escherichia coli Proteins, Evolution, Molecular, Iron, Open Reading Frames, Phylogeny, Pseudogenes, Selection, Genetic