MnO nanosheets as the biomimetic oxidase for rapid and sensitive oxalate detection combining with bionic E-eye.

Ying Gan, Ning Hu, Chuanjiang He, Shuqi Zhou, Jiawei Tu, Tao Liang, Yuxiang Pan, Dmitry Kirsanov, Andrey Legin, Hao Wan, Ping Wang

Journal: Biosensors & bioelectronics 2019;130():254-261

PMID: 30771714

Full Text on Elsevier Science View on PubMed

Abstract

Urolithiasis commonly occurs in kidney and ureteral, and may cause local organ/tissue damage, even kidney failure. The incidence of this disease is increasing worldwide, in which calcium oxalate is the major composition forming the urinary calculus. Therefore, to monitor this disease for the prevention and treatment, measuring the oxalate in the urine is of great significance. Here, a rapid and sensitive colorimetric method was developed based on 3,3',5,5'-tetramethylbenzidine-manganese dioxide (TMB-MnO) nanosheets for oxalate detection. MnO nanosheets acted as an efficient biomimetic oxidase to catalyze the reaction with TMB and oxalate. Pale yellow TMB can be oxidized to blue oxide TMB catalyzed by BSA-stabilized MnO nanosheets, and oxalate can selectively inhibit this reaction by consuming and reacting with MnO nanosheets, thus achieving the quantitative detection of oxalate. Moreover, a home-made bionic electronic-eye (E-eye) system was developed as a portable in-situ detection platform to efficiently measure the oxalate concentrations in 10 s by direct photographing. By optimizing experimental conditions, this method shows a wide linear range (7.8 μM to 250 μM) and a low detection limit (0.91 μM) for oxalate detection. Besides, this method exhibits high selectivity even with 80-fold interfering chemicals. Furthermore, the performance of the method was validated by testing the artificial urine samples, indicating its great potential for monitoring and diagnosis of urolithiasis in point-of-care applications.

Address: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China.; Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China.; Laboratory of Chemical Sensors, Chemistry Department, Mendeleev Center, Saint-Petersburg State University, Saint-Petersburg 199034, Russia.; Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: [email protected].; Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: [email protected].

DOI: 10.1016/j.bios.2019.01.026

Link outs

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S0956...

MeSH Terms: Biomimetics, Bionics, Biosensing Techniques, Colorimetry, Glutathione, Humans, Limit of Detection, Nanostructures, Oxalates, Oxidation-Reduction, Oxidoreductases