Extracts from Myrtle Liqueur Processing Waste Modulate Stem Cells Pluripotency under Stressing Conditions.

Giorgia Sarais, Maurizio Mulas, Carlo Ventura, Sara Cruciani, Sara Santaniello, Angela Fadda, Luana Sale, Daniele Sanna, Giorgio Carlo Ginesu, Maria Laura Cossu, Pier Andrea Serra, Margherita Maioli

Journal: BioMed research international 2019;2019():5641034

PMID: 31309107

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Abstract

Nutraceuticals present in food are molecules able to exert biological activity for the prevention and treatment of various diseases, in form of pharmaceutical preparations, such as capsules, cream, or pills. L. is a spontaneous Mediterranean evergreen shrub, widely known for the liqueur obtained from its berries rich in phytochemicals such as tannins and flavonoids. In the present study, we aimed to evaluate the properties of myrtle byproducts, residual of the industrial liqueur processing, in Adipose-derived stem cells (ADSCs) induced at oxidative stress by in vitro HO treatment. Cells were exposed for 12-24 and 48h at treatment with extracts and then senescence-induced. ROS production was then determined. The real-time PCR was performed to evaluate the expression of inflammatory cytokines and sirtuin-dependent epigenetic changes, as well the modifications in terms of stem cell pluripotency. The -galactosidase assay was conducted to analyze stem cell senescence after treatment. Our results show that industrial myrtle byproducts retain a high antioxidant and antisenescence activity, protecting cells from oxidative stress damages. The results obtained suggest that residues from myrtle liqueur production could be used as resource in formulation of food supplements or pharmaceutical preparations with antioxidant, antiaging, and anti-inflammatory activity.

Address: Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.; Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems-Eldor Lab, Innovation Accelerator, CNR, Via Piero Gobetti 101, 40129 Bologna, Italy.; Instituto di Scienze delle Produzioni Alimentari (ISPA), Consiglio Nazionale delle Ricerche (CNR), Traversa la Crucca 3, 07100 Sassari, Italy.; Department of Agriculture, University of Sassari, Via De Nicola 9, 07100 Sassari, Italy.; Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.; Institute of Biomolecular Chemistry (ICB), Consiglio Nazionale delle Ricerche (CNR), Traversa la Crucca 3, 07100 Sassari, Italy.; General Surgery Unit 2 "Clinica Chirurgica" Medical, Surgical and Experimental Sciences Department, University of Sassari, Viale San Pietro 8, 07100 Sassari, Italy.; Department of Clinical and Experimental Medicine, University of Sassari, Viale S. Pietro 43/b, 07100 Sassari, Italy.; Department of Biomedical Sciences, Center for Developmental Biology and Reprogramming (CEDEBIOR), University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.; Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche (CNR), Monserrato, Cagliari, Italy.

DOI: 10.1155/2019/5641034

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MeSH Terms: Anti-Inflammatory Agents, Antioxidants, Cytokines, Female, Flavonoids, Humans, Inflammation, Male, Middle Aged, Myrtus, Oxidative Stress, Plant Extracts, Reactive Oxygen Species, Stem Cells, Tannins, beta-Galactosidase