Basic Phytochrome B Calculations.

Robert W Smith, Christian Fleck

Journal: Methods in molecular biology (Clifton, N.J.) 2020;2026():121-133

PMID: 31317407

Abstract

Mathematical models are important tools in helping us to understand complex biological systems. Models of phytochrome-regulated systems in Arabidopsis thaliana have shown the importance of dimerization, nuclear transport, and thermal/dark reversion in mediating phytochrome activity and plant development. Here we go through the steps required to calculate the steady-state amounts of phytochrome subspecies relative to the total phytochrome molecule population. Starting from a simplified two-state system we expand and apply the technique to the extended phytochrome dimer model. Additionally, we provide a Python package that can automatically calculate the proportion of phytochrome B in a particular state given specific experimental conditions.

Address: Laboratory of Systems and Synthetic Biology, Wageningen UR, Wageningen, The Netherlands. [email protected].; Sir William Dunn School of Pathology, University of Oxford, Oxford, UK. [email protected].; Laboratory of Systems and Synthetic Biology, Wageningen UR, Wageningen, The Netherlands. [email protected].; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland. [email protected].

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