Impaired L-arginine uptake in platelets from type-2 diabetic patients.

M G Signorello, M Giovine, R Pascale, L Cataldi, C Bordone, U Benatti, G Leoncini

Journal: Biotechnology and applied biochemistry 2001;34(1):19-23

PMID: 11483150

Abstract

The L-arginine uptake of resting platelets from type-2 diabetic patients and control subjects was measured and the kinetic parameters defined. The effect of platelet stimulation with agonists was also investigated. Kinetic studies showed that the K(m) value for L-arginine transport was not different in patients compared with control subjects, while V(max) was significantly decreased in patients compared with controls. Moreover, agonists able to mobilize Ca(2+) produced a further decrease in the L-arginine uptake in controls and in patients, suggesting that Ca(2+) intracellular levels down-regulate L-arginine transport in platelets from both control subjects and patients. Data suggest that drugs designed to control intracellular Ca(2+) might restore platelet function in diabetic patients.

Address: Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV 1, 16132 Genova, Italy.

DOI: 10.1042/ba20010022

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MeSH Terms: Aged, Arginine, Blood Platelets, Calcimycin, Calcium, Diabetes Mellitus, Type 2, Dose-Response Relationship, Drug, Down-Regulation, Female, Humans, Kinetics, Male, Middle Aged, Nitric Oxide, Time Factors