Experimental Woodsmoke Exposure During Exercise and Blood Oxidative Stress.

Bridget Peters, Christopher Ballmann, Tiffany Quindry, Emily G Zehner, Justin McCroskey, Matthew Ferguson, Tony Ward, Charles Dumke, John C Quindry

Journal: Journal of occupational and environmental medicine 2019;60(12):1073-1081

PMID: 30188494

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Abstract

OBJECTIVES

The current laboratory study quantified blood oxidative stress to woodsmoke exposure.

METHODS

Participants inhaled woodsmoke during three randomized crossover exercise trials (Clean Air [0 μg/m], Low Exposure [250 μg/m], and High Exposure [500 μg/m], Woodsmoke [particulate matter less than 2.5 μm, PM2.5]). Trolox equivalent antioxidant capacity (TEAC), uric acid (UA), 8-isoprostanes (8-ISO), lipid hydroperoxides (LOOH), protein carbonyls (PC), nitrotyrosine (3-NT), 8-isoprostane, and myeloperoxidase (MPO) were quantified in Pre, immediately Post, and 1- (1Hr) hour post blood samples.

RESULTS

UA decreased following Low Exposure, while plasma TEAC levels increased Post and 1Hr. LOOH levels decreased 1Hr Post (High Exposure), while 8-Iso increased following both smoke trials. PC and MPO were unchanged following all trials, while 3-NT increased over Clean Air.

CONCLUSION

Blood oxidative stress occurred largely independent of PM2.5 concentrations. Future studies should employ longer duration smoke and exercise combined with physiologic parameters.

Address: School of Kinesiology, Auburn University, Auburn, Alabama (Dr Peters, Dr Ballmann, Ms Zehner, Mr McCroskey); Department of Health and Human Performance (Ms Quindry, Dr Dumke, Dr Quindry); Center for Environmental Health Sciences (Mr Ferguson); School of Public and Community Health Sciences (Dr Ward), University of Montana, Missoula, Montana.

DOI: 10.1097/JOM.0000000000001437

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MeSH Terms: Adult, Antioxidants, Cross-Over Studies, Dinoprost, Exercise Test, Humans, Inhalation Exposure, Isoprostanes, Lipid Peroxides, Oxidative Stress, Particulate Matter, Peroxidase, Physical Exertion, Protein Carbonylation, Random Allocation, Smoke, Tyrosine, Uric Acid, Wildfires, Wood, Young Adult