Increased carbohydrate production from carbon dioxide in randomly mutated cells of cyanobacterial strain Synechocystis sp. PCC 6714: Bioprocess understanding and evaluation of productivities.

Donya Kamravamanesh, Christoph Slouka, Andreas Limbeck, Maximilian Lackner, Christoph Herwig

Journal: Bioresource technology 2019;273():277-287

PMID: 30448679

Full Text on Elsevier Science View on PubMed

Abstract

Recently, several mutants of Synechocystis sp. PCC 6714 were obtained showing superior PHB content and productivities. Here, the most promising mutant named MT_a24 is compared in detail with the wild-type in controlled photobioreactors. In order to provide an easily scalable and alternative approach to the normally done two-step process -comprising of growth phase and limitation phase- a one-step cultivation was optimized. The multivariate experimental design approach was used for the optimization of the one-step, self-limiting media. During one-step cultivation of MT_a24 with optimized media 30 ± 4% (DCW) corresponding to 1.16 g L PHB was obtained. Using pulse experiments it was demonstrated that phosphate is the key driver of glycogen synthesis in Synechocystis sp. PCC 6714 and it can be used to boost glycogen productivity. The maximum glycogen content acquired was 2.6 g L (76.2% DCW) for mutant MT_a24 using phosphate feeding and carbon dioxide as carbon source.

Address: Institute of Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, 1060 Vienna, Austria; Institute of Chemical Technologies and Analytics, Technische Universität Wien, 1060 Vienna, Austria. Electronic address: [email protected].; Institute of Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, 1060 Vienna, Austria; CD Laboratory on Mechanistic and Physiological Methods for Improved Bioprocesses, Technische Universität Wien, 1060 Vienna, Austria. Electronic address: [email protected].; Institute of Chemical Technologies and Analytics, Technische Universität Wien, 1060 Vienna, Austria. Electronic address: [email protected].; Lackner Ventures & Consulting GmbH, Hofherr Schrantz Gasse 2, 1210 Vienna, Austria; University of Applied Sciences FH Technikum Wien, 1200 Vienna, Austria. Electronic address: [email protected].; Institute of Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, 1060 Vienna, Austria; CD Laboratory on Mechanistic and Physiological Methods for Improved Bioprocesses, Technische Universität Wien, 1060 Vienna, Austria. Electronic address: [email protected].

DOI: 10.1016/j.biortech.2018.11.025

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MeSH Terms: Carbohydrate Metabolism, Carbohydrates, Carbon, Carbon Dioxide, Glycogen, Photobioreactors, Synechocystis