Nicotinamide-Containing Di- and Trinucleotides as Chemical Tools for Studies of NAD-Capped RNAs.

Agnieszka Mlynarska-Cieslak, Anais Depaix, Ewa Grudzien-Nogalska, Pawel J Sikorski, Marcin Warminski, Megerditch Kiledjian, Jacek Jemielity, Joanna Kowalska

Journal: Organic letters 2019;20(23):7650-7655

PMID: 30479128

Full Text on American Chemical Society View on PubMed

Abstract

We report the chemical synthesis of a set of nicotinamide adenine dinucleotide (NAD) cap analogues containing chemical modifications that reduce their susceptibility to NAD-RNA-degrading enzymes. These analogues can be incorporated into transcripts in a similar way as NAD. Biochemical characterization of RNAs carrying these caps with DXO, NudC, and Nudt12 enzymes led to the identification of compounds that can be instrumental in unraveling so far unaddressed biological aspects of NAD-RNAs.

Address: Division of Biophysics, Institute of Experimental Physics, Faculty of Physics , University of Warsaw , Pasteura 5 , 02-093 Warsaw , Poland.; Department of Cell Biology and Neuroscience , Rutgers University , Piscataway , New Jersey 08854 , United States.; Centre of New Technologies , University of Warsaw , Banacha 2c , 02-097 Warsaw , Poland.

DOI: 10.1021/acs.orglett.8b03386

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https://doi.org/10.1021/acs.orglett.8b03386

MeSH Terms: Adenine, Molecular Conformation, NAD, Niacinamide, RNA Caps