Isolation of C-phycocyanin from Spirulina platensis microalga using Ionic liquid based aqueous two-phase system.

Yu-Kaung Chang, Pau-Loke Show, John Chi-Wei Lan, Jung-Chin Tsai, Chi-Rong Huang

Journal: Bioresource technology 2019;270():320-327

PMID: 30241065

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Abstract

An aqueous two-phase system (ATPS) with ionic liquids (ILs) was used for the isolate of C-phycocyanin (CPC) from Spirulina platensis microalga. Various imidazolium ILs and potassium salts were studied. The effect of ILs-ATPS on the extraction efficiency of CPC was also studied. The experimental parameters like pH, loading volume, algae concentration, temperature, and alkyl chain length of IL were well-covered in this report. The experimental results showed that the extraction efficiency, the partition coefficient, and the separation factor for CPC were 99%, 36.6, and 5.8, respectively, for an optimal pH value of 7 and a temperature of 308 K. The order of extraction efficiency for CPC using IL-ATPS was: 1-octyl-3-methylimidazolium bromide (C8MIM-Br) > 1-hexyl-3-methylimidazolium bromide (C6MIM-Br) > 1-butyl-3-methylimidazolium bromide (C4MIM-Br). The isolation process followed the pseudo second-order kinetic model and the thermodynamic results were obviously spontaneous.

Address: Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan. Electronic address: [email protected].; Bioseparation Research Group, Department of Chemical Engineering and Environmental Engineering, University of Nottingham Malaysia Campus, Selangor Darul Ehsan, Malaysia.; Department of Chemical Engineering and Materials Science, College of Engineering, Yuan Ze University, Taiwan.; Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan.

DOI: 10.1016/j.biortech.2018.07.138

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MeSH Terms: Borates, Imidazoles, Ionic Liquids, Microalgae, Phycocyanin, Spirulina, Temperature, Thermodynamics