Advances in the Application of Modified Nucleotides in SELEX Technology.

O M Antipova, E G Zavyalova, A V Golovin, G V Pavlova, A M Kopylov, R V Reshetnikov

Journal: Biochemistry. Biokhimiia 2018;83(10):1161-1172

PMID: 30472954

Full Text on Biochemistry (Moscow) View on PubMed

Abstract

Aptamers are widely used as molecular recognition elements for detecting and blocking functional biological molecules. Since the common "alphabet" of DNA and RNA consists of only four letters, the chemical diversity of aptamers is less than the diversity of protein recognition elements built of 20 amino acids. Chemical modification of nucleotides enlarges the potential of DNA/RNA aptamers. This review describes the latest achievements in a variety of approaches to aptamers selection with an extended genetic alphabet.

Address: Lomonosov Moscow State University, Faculty of Chemistry, Moscow, 119991, Russia. [email protected].; Apto-Pharm Ltd., Moscow, 115564, Russia.; Lomonosov Moscow State University, Faculty of Chemistry, Moscow, 119991, Russia.; Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow, 119234, Russia.; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, 119991, Russia.; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia.; Burdenko National Scientific and Practical Center for Neurosurgery, Ministry of Healthcare of the Russian Federation, Moscow, 125047, Russia.

DOI: 10.1134/S0006297918100024

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https://doi.org/10.1134/S0006297918100024

MeSH Terms: Amino Acids, Aptamers, Nucleotide, Base Pairing, Click Chemistry, Deoxyribose, Nucleotidases, Oligonucleotides, SELEX Aptamer Technique