Genome-wide association meta-analysis of age at first cannabis use.
Camelia C Minică, Karin J H Verweij, Peter J van der Most, Hamdi Mbarek, Manon Bernard, Kristel R van Eijk, Penelope A Lind, Meng Zhen Liu, Dominique F Maciejewski, Teemu Palviainen, Cristina Sánchez-Mora, Richard Sherva, Michelle Taylor, Raymond K Walters, Abdel Abdellaoui, Timothy B Bigdeli, Susan J T Branje, Sandra A Brown, Miguel Casas, Robin P Corley, George Davey-Smith, Gareth E Davies, Erik A Ehli, Lindsay Farrer, Iryna O Fedko, Iris Garcia-Martínez, Scott D Gordon, Catharina A Hartman, Andrew C Heath, Ian B Hickie, Matthew Hickman, Christian J Hopfer, Jouke Jan Hottenga, René S Kahn, Jaakko Kaprio, Tellervo Korhonen, Henry R Kranzler, Ken Krauter, Pol A C van Lier, Pamela A F Madden, Sarah E Medland, Michael C Neale, Wim H J Meeus, Grant W Montgomery, Ilja M Nolte, Albertine J Oldehinkel, Zdenka Pausova, Josep A Ramos-Quiroga, Vanesa Richarte, Richard J Rose, Jean Shin, Michael C Stallings, Tamara L Wall, Jennifer J Ware, Margaret J Wright, Hongyu Zhao, Hans M Koot, Tomas Paus, John K Hewitt, Marta Ribasés, Anu Loukola, Marco P Boks, Harold Snieder, Marcus R Munafò, Joel Gelernter, Dorret I Boomsma, Nicholas G Martin, Nathan A Gillespie, Jacqueline M Vink, Eske M Derks
Journal: Addiction (Abingdon, England)
2019;113(11):2073-2086
PMID: 30003630
Abstract
BACKGROUND AND AIMS
Cannabis is one of the most commonly used substances among adolescents and young adults. Earlier age at cannabis initiation is linked to adverse life outcomes, including multi-substance use and dependence. This study estimated the heritability of age at first cannabis use and identified associations with genetic variants.
METHODS
A twin-based heritability analysis using 8055 twins from three cohorts was performed. We then carried out a genome-wide association meta-analysis of age at first cannabis use in a discovery sample of 24 953 individuals from nine European, North American and Australian cohorts, and a replication sample of 3735 individuals.
RESULTS
The twin-based heritability for age at first cannabis use was 38% [95% confidence interval (CI) = 19-60%]. Shared and unique environmental factors explained 39% (95% CI = 20-56%) and 22% (95% CI = 16-29%). The genome-wide association meta-analysis identified five single nucleotide polymorphisms (SNPs) on chromosome 16 within the calcium-transporting ATPase gene (ATP2C2) at P < 5E-08. All five SNPs are in high linkage disequilibrium (LD) (r > 0.8), with the strongest association at the intronic variant rs1574587 (P = 4.09E-09). Gene-based tests of association identified the ATP2C2 gene on 16q24.1 (P = 1.33e-06). Although the five SNPs and ATP2C2 did not replicate, ATP2C2 has been associated with cocaine dependence in a previous study. ATP2B2, which is a member of the same calcium signalling pathway, has been associated previously with opioid dependence. SNP-based heritability for age at first cannabis use was non-significant.
CONCLUSION
Age at cannabis initiation appears to be moderately heritable in western countries, and individual differences in onset can be explained by separate but correlated genetic liabilities. The significant association between age of initiation and ATP2C2 is consistent with the role of calcium signalling mechanisms in substance use disorders.
© 2018 Society for the Study of Addiction.
Address:
Department of Biological Psychology/Netherlands Twin Register, VU University, Amsterdam, the Netherlands.; Department of Biological Psychology/Netherlands Twin Register, VU University, Amsterdam, the Netherlands.; Behavioral Science Institute, Radboud University, Nijmegen, the Netherlands.; Amsterdam UMC, University of Amsterdam, Department of Psychiatry, Amsterdam, the Netherlands.; Department of Epidemiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.; Hospital for Sick Children Research Institute, Toronto, Canada.; Department of Neurology, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, the Netherlands.; Psychiatric Genetics, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.; Institute for Behavioral Genetics, Department of Psychology and Neuroscience, University of Colorado Boulder, Boulder, CO, USA.; Department of Clinical Developmental Psychology, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.; GGZ inGeest and Department of Psychiatry, Amsterdam Public Health Research Institute, VU University Medical Center, Amsterdam, the Netherlands.; Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland.; Psychiatric Genetics Unit, Group of Psychiatry, Mental Health and Addiction, Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.; Department of Psychiatry, Hospital Universitari Vall d'Hebron, Barcelona, Spain.; Biomedical Network Research Centre on Mental Health (CIBERSAM), Instituto de Salud Carlos III, Madrid, Spain.; Biomedical Genetics Department, Boston University School of Medicine, Boston, MA, USA.; MRC Integrative Epidemiology Unit (IEU), University of Bristol, Bristol, UK.; School of Social and Community Medicine, University of Bristol, Bristol, UK.; Analytic and Translational Genetics Unit, Massachusetts General Hospital, Boston, MA, USA.; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Medicine, Harvard Medical School, Boston, MA, USA.; Department of Biological Psychology/Netherlands Twin Register, VU University, Amsterdam, the Netherlands.; Amsterdam UMC, University of Amsterdam, Department of Psychiatry, Amsterdam, the Netherlands.; Department of Psychiatry, Virginia Institute for Psychiatric and Behavior Genetics, Virginia Commonwealth University, Richmond, VA, USA.; Research Centre Adolescent Development, Utrecht University, Utrecht, the Netherlands.; Department of Psychology and Psychiatry, University of California San Diego, La Jolla, CA, USA.; Psychiatric Genetics Unit, Group of Psychiatry, Mental Health and Addiction, Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.; Department of Psychiatry, Hospital Universitari Vall d'Hebron, Barcelona, Spain.; Biomedical Network Research Centre on Mental Health (CIBERSAM), Instituto de Salud Carlos III, Madrid, Spain.; Department of Psychiatry and Legal Medicine, Universitat Autònoma de Barcelona, Barcelona, Spain.; Avera Institute for Human Genetics, Sioux Falls, SD, USA.; Department of Medicine (Biomedical Genetics), Boston University School of Medicine, Boston, MA, USA.; Psychiatric Genetics Unit, Group of Psychiatry, Mental Health and Addiction, Vall d'Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain.; Department of Psychiatry, Hospital Universitari Vall d'Hebron, Barcelona, Spain.; Genetic Epidemiology, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.; Department of Psychiatry, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.; Department of Psychiatry, Washington University School of Medicine, St Louis, MO, USA.; Brain and Mind Research Institute, University of Sydney, Sydney, NSW, Australia.; School of Social and Community Medicine, University of Bristol, Bristol, UK.; Department of Psychiatry, University of Colorado Denver, Aurora, CO, USA.; Department of Psychiatry, Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, the Netherlands.; Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland.; Department of Public Health, University of Helsinki, Helsinki, Finland.; Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Helsinki, Finland.; University of Eastern Finland, Institute of Public Health and Clinical Nutrition, Kuopio, Finland.; Department of Psychiatry, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA.; Department of Clinical Developmental Psychology, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.; Department of Psychology, Education and Child Studies, Erasmus University Rotterdam, Rotterdam, the Netherlands.; Department of Psychiatry and School of Medicine, Virginia Commonwealth University, Richmond, VA, USA.; Research Centre Adolescent Development, Utrecht University, Utrecht, the Netherlands.; Developmental Psychology, Tilburg University, Tilburg, the Netherlands.; Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.; Hospital for Sick Children Research Institute, Toronto, Canada.; Physiology and Nutritional Sciences, University of Toronto, Toronto, Canada.; Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, USA.; Department of Psychiatry, University of California San Diego, La Jolla, CA, USA.; Queensland Brain Institute, The University of Queensland, Brisbane, Queensland, Australia.; Department of Biostatistics, Yale School of Public Health and VA CT, New Haven, CT, USA.; Department of Clinical Developmental Psychology, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.; Rotman Research Institute, Baycrest, Toronto, Canada.; Psychology and Psychiatry, University of Toronto, Toronto, Canada.; Center for the Developing Brain, Child Mind Institute, New York, NY, USA.; MRC Integrative Epidemiology Unit (IEU), University of Bristol, Bristol, UK.; UK Centre for Tobacco and Alcohol Studies, School of Experimental Psychology, University of Bristol, Bristol, UK.; Psychiatry, Genetics, and Neuroscience, Yale University School of Medicine and VA CT, West Haven, CT, USA.; Department of Psychiatry, Virginia Institute for Psychiatric and Behavior Genetics, Virginia Commonwealth University, Richmond, VA, USA.; Genetic Epidemiology, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.; Behavioral Science Institute, Radboud University, Nijmegen, the Netherlands.; Department of Psychiatry, Academic Medical Centre, Amsterdam, the Netherlands.; Translational Neurogenomics group, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
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MeSH Terms:
Adolescent,
Adult,
Age of Onset,
Calcium-Transporting ATPases,
Female,
Genome-Wide Association Study,
Humans,
Male,
Marijuana Use,
Middle Aged,
Polymorphism, Single Nucleotide,
Twins,
Young Adult