25-Hydroxyvitamin D Testing: Immunoassays Versus Tandem Mass Spectrometry.

Uttam Garg

Journal: Clinics in laboratory medicine 2019;38(3):439-453

PMID: 30115390

Full Text on Elsevier Science View on PubMed

Abstract

Vitamin D has been associated with many health conditions. Because of widespread deficiency in the general population, laboratory testing of vitamin D has increased exponentially in recent years. Currently, 25-hydroxyvitamin D (25[OH]D) is considered the best marker of vitamin D status. Automated immunoassays and tandem mass spectrometry are the most widely used assays for the measurement of 25(OH)D. Because a medical decision of vitamin D deficiency and treatment are made based on specific levels, it is important that different 25(OH)D assays are harmonized. Despite standardization efforts, significant differences remain among various methods and laboratories for the measurement of 25(OH)D.

Address: Department of Pathology and Laboratory Medicine, Children's Mercy Hospital, University of Missouri School of Medicine, 2401 Gillham Road, Kansas City, MO 64108, USA. Electronic address: [email protected].

DOI: 10.1016/j.cll.2018.05.007

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MeSH Terms: Chromatography, High Pressure Liquid, Clinical Chemistry Tests, Humans, Immunoassay, Tandem Mass Spectrometry, Vitamin D