Donor Site Location Is Critical for Proliferation, Stem Cell Capacity, and Osteogenic Differentiation of Adipose Mesenchymal Stem/Stromal Cells: Implications for Bone Tissue Engineering.

Marie K Reumann, Caren Linnemann, Romina H Aspera-Werz, Sigrid Arnold, Manuel Held, Claudine Seeliger, Andreas K Nussler, Sabrina Ehnert

Journal: International journal of molecular sciences 2018;19(7):1868

PMID: 29949865

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Abstract

Human adipose mesenchymal stem/stromal cells (Ad-MSCs) have been proposed as a suitable option for bone tissue engineering. However, donor age, weight, and gender might affect the outcome. There is still a lack of knowledge of the effects the donor tissue site might have on Ad-MSCs function. Thus, this study investigated proliferation, stem cell, and osteogenic differentiation capacity of human Ad-MSCs obtained from subcutaneous fat tissue acquired from different locations (abdomen, hip, thigh, knee, and limb). Ad-MSCs from limb and knee showed strong proliferation despite the presence of osteogenic stimuli, resulting in limited osteogenic characteristics. The less proliferative Ad-MSCs from hip and thigh showed the highest alkaline phosphatase (AP) activity and matrix mineralization. Ad-MSCs from the abdomen showed good proliferation and osteogenic characteristics. Interestingly, the observed differences were not dependent on donor age, weight, or gender, but correlated with the expression of , , , and . Especially, low basal levels seemed to be pivotal for osteogenic differentiation. Our data clearly show that the donor tissue site affects the proliferation and osteogenic differentiation of Ad-MSCs significantly. Thus, for bone tissue engineering, the donor site of the adipose tissue from which the Ad-MSCs are derived should be adapted depending on the requirements, e.g., cell number and differentiation state.

Address: Department of Trauma and Reconstructive Surgery, Siegfried Weller Research Institute, Eberhard Karls University Tuebingen, BG Trauma Center Tuebingen, 72076 Tuebingen, Germany. [email protected].; Department of Trauma and Reconstructive Surgery, Siegfried Weller Research Institute, Eberhard Karls University Tuebingen, BG Trauma Center Tuebingen, 72076 Tuebingen, Germany. [email protected].; Department of Trauma and Reconstructive Surgery, Siegfried Weller Research Institute, Eberhard Karls University Tuebingen, BG Trauma Center Tuebingen, 72076 Tuebingen, Germany. [email protected].; Department of Trauma and Reconstructive Surgery, Siegfried Weller Research Institute, Eberhard Karls University Tuebingen, BG Trauma Center Tuebingen, 72076 Tuebingen, Germany. [email protected].; Department of Trauma and Reconstructive Surgery, Siegfried Weller Research Institute, Eberhard Karls University Tuebingen, BG Trauma Center Tuebingen, 72076 Tuebingen, Germany. [email protected].; Department of Hand, Plastic, Reconstructive and Aesthetic Surgery, Eberhard Karls University Tuebingen, BG Trauma Center Tuebingen, 72076 Tuebingen, Germany. [email protected].; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, 85354 Freising, Germany. [email protected].; Experimental Trauma Surgery, Klinikum Rechts der Isar, Technical University of Munich, 81675 Munich, Germany. [email protected].; Department of Trauma and Reconstructive Surgery, Siegfried Weller Research Institute, Eberhard Karls University Tuebingen, BG Trauma Center Tuebingen, 72076 Tuebingen, Germany. [email protected].; Department of Trauma and Reconstructive Surgery, Siegfried Weller Research Institute, Eberhard Karls University Tuebingen, BG Trauma Center Tuebingen, 72076 Tuebingen, Germany. [email protected].

DOI: 10.3390/ijms19071868

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MeSH Terms: Adipose Tissue, Alkaline Phosphatase, Antigens, CD, Bone Matrix, Bone and Bones, Calcification, Physiologic, Cell Differentiation, Cell Proliferation, Core Binding Factor Alpha 1 Subunit, Female, Hip, Humans, Male, Mesenchymal Stem Cells, Middle Aged, Osteogenesis, Sp7 Transcription Factor, Thigh, Tissue Donors, Tissue Engineering