Determination of the binding properties of p-cresyl glucuronide to human serum albumin.

Dan Yi, Elisa Bernardes Monteiro, Stéphane Chambert, Hédi A Soula, Julio B Daleprane, Christophe O Soulage

Journal: Biochimie 2018;150():1-7

PMID: 29705133

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Abstract

p-Cresyl glucuronide (p-CG) is a by-product of tyrosine metabolism that accumulates in patients with end-stage renal disease. p-CG binding to human serum albumin in physiological conditions (37 °C, pH 7.40) was studied by ultrafiltration (MWCO 10 kDa) and data were analyzed assuming one binding site. The estimated value of the association constant was 2.77 × 10 M and a maximal stoichiometry of 3.80 mol per mole. At a concentration relevant for end-stage renal patients, p-CG was 23% bound to albumin. Competition experiments, using fluorescent probes, demonstrated that p-CG did not bind to Sudlow's site I or site II. The p-CG did not interfere with the binding of p-cresyl-sulfate or indoxyl sulfate to serum albumin.

Address: Univ. Lyon, CarMeN, INSERM U1060, INRA U1397, INSA de Lyon, Université Claude Bernard Lyon 1, F-69621 Villeurbanne, France.; Univ. Lyon, CarMeN, INSERM U1060, INRA U1397, INSA de Lyon, Université Claude Bernard Lyon 1, F-69621 Villeurbanne, France; Laboratory for Studies of Interactions Between Nutrition and Genetics, LEING, Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.; INSA Lyon, ICBMS, Laboratoire de Chimie Organique et Bioorganique, Bât J. Verne, 20 av A. Einstein, 69621 Villeurbanne Cedex, France.; Sorbonne Universités, Univ. Pierre et Marie Curie - Paris 6, 75006 Paris, France.; Laboratory for Studies of Interactions Between Nutrition and Genetics, LEING, Department of Basic and Experimental Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.; Univ. Lyon, CarMeN, INSERM U1060, INRA U1397, INSA de Lyon, Université Claude Bernard Lyon 1, F-69621 Villeurbanne, France. Electronic address: [email protected].

DOI: 10.1016/j.biochi.2018.04.019

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MeSH Terms: Cresols, Glucuronides, Humans, Protein Binding, Serum Albumin, Human