Untargeted Metabolomic Screen Reveals Changes in Human Plasma Metabolite Profiles Following Consumption of Fresh Broccoli Sprouts.

Lauren Housley, Armando Alcazar Magana, Anna Hsu, Laura M Beaver, Carmen P Wong, Jan F Stevens, Jaewoo Choi, Yuan Jiang, Deborah Bella, David E Williams, Claudia S Maier, Jackilen Shannon, Roderick H Dashwood, Emily Ho

Journal: Molecular nutrition & food research 2019;62(19):e1700665

PMID: 29377494

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Abstract

SCOPE

Several lines of evidence suggest that the consumption of cruciferous vegetables is beneficial to human health. Yet, underlying mechanisms and key molecular targets that are involved with achieving these benefits in humans are still not fully understood. To accelerate this research, we conduct a human study to identify potential molecular targets of crucifers for further study. This study aims to characterize plasma metabolite profiles in humans before and after consuming fresh broccoli sprouts (a rich dietary source of bioactive sulforaphane).

METHODS AND RESULTS

Ten healthy adults consume fresh broccoli sprouts (containing 200 μmol sulforaphane equivalents) at time 0 and provide blood samples at 0, 3, 6, 12, 24, and 48 h. An untargeted metabolomics screen reveals that levels of several plasma metabolites are significantly different before and after sprout intake, including fatty acids (14:0, 14:1, 16:0, 16:1, 18:0, and 18:1), glutathione, glutamine, cysteine, dehydroepiandrosterone, and deoxyuridine monophosphate. Evaluation of all time points is conducted using paired t-test (R software) and repeated measures analysis of variance for a within-subject design (Progenesis QI).

CONCLUSION

This investigation identifies several potential molecular targets of crucifers that may aid in studying established and emerging health benefits of consuming cruciferous vegetables and related bioactive compounds.

Address: School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR, USA.; Department of Nutrition and Food Science, California State University, Chico, CA, USA.; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA.; Department of Chemistry, Oregon State University, Corvallis, OR, USA.; School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR, USA.; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA.; Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, USA.; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA.; Department of Statistics, Oregon State University, Corvallis, OR, USA.; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA.; Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA.; Department of Chemistry, Oregon State University, Corvallis, OR, USA.; Department of Public Health & Preventive Medicine, Oregon Health and Science University, Portland, OR, USA.; Center for Epigenetics & Disease Prevention, Texas A&M Health Science Center, Houston, TX, USA.; School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR, USA.; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA.; Center for Epigenetics & Disease Prevention, Texas A&M Health Science Center, Houston, TX, USA.; Moore Family Center for Whole Grain Foods, Nutrition and Preventive Health, Oregon State University, Corvallis, OR, USA.

DOI: 10.1002/mnfr.201700665

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MeSH Terms: Adult, Blood, Brassica, Dehydroepiandrosterone, Deoxyuracil Nucleotides, Fatty Acids, Female, Glutathione, Humans, Isothiocyanates, Male, Metabolomics, Middle Aged, Sulfoxides