Prostaglandin E (PGE) is a testicular peritubular cell-derived factor involved in human testicular homeostasis.

Verónica Rey-Ares, Soledad Paola Rossi, Kim-Gwendolyn Dietrich, Frank-Michael Köhn, J Ullrich Schwarzer, Harald Welter, Mónica Beatriz Frungieri, Artur Mayerhofer

Journal: Molecular and cellular endocrinology 2019;473():217-224

PMID: 29408603

Full Text on Elsevier Science View on PubMed

Abstract

In man, blockage of prostaglandin (PG)-production e.g. by non-steroidal anti-inflammatory drug (NSAIDs) may have negative testicular side effects, implying beneficial actions of PGs in the testis. We examined human testicular samples and isolated human testicular peritubular cells (HTPCs) to explore sites of PG-synthesis and targets. HTPCs express cyclooxygenase 1 (COX1) and secrete PGE. Receptors (EP1, 2, 4) were specifically identified in peritubular cells. In HTPCs PGE significantly increased mRNA levels of the contractility protein calponin, but did not induce contractions. PGE, as well as EP1 and EP4 receptor agonists, significantly increased glia cell line derived neurotrophic factor (GDNF) mRNA and/or protein levels. Importantly, the NSAID ibuprofen reduced PGE and this action also lowered SMA and calponin mRNA levels and levels of secreted GDNF protein. The results reveal an unknown PGE system in the human testis, in involving peritubular cells, which may be prone to interference by NSAIDs.

Address: Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany. Electronic address: [email protected].; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany; Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. Electronic address: [email protected].; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany. Electronic address: [email protected].; Andrologicum, D-80331 Munich, Germany. Electronic address: [email protected].; Andrology-Center, D-81241 Munich, Germany. Electronic address: [email protected].; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany. Electronic address: [email protected].; Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. Electronic address: [email protected].; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Ludwig-Maximilian-University (LMU), D-82152 Planegg, Germany. Electronic address: [email protected].

DOI: 10.1016/j.mce.2018.01.022

Link outs

Free resources

Other Literature Sources:

https://scite.ai/reports/29408603

Miscellaneous:

https://assays.cancer.gov/non-CPTAC-3327

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S0303...

MeSH Terms: Actins, Biomarkers, Calcium-Binding Proteins, Cyclooxygenase 1, Dinoprostone, Glial Cell Line-Derived Neurotrophic Factor, Homeostasis, Humans, Ibuprofen, Male, Microfilament Proteins, Muscle Contraction, RNA, Messenger, Receptors, Prostaglandin E, EP4 Subtype, Testis, Calponins