Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts.

Nassima Boutaoui, Lahcene Zaiter, Fadila Benayache, Samir Benayache, Simone Carradori, Stefania Cesa, Anna Maria Giusti, Cristina Campestre, Luigi Menghini, Denise Innosa, Marcello Locatelli

Journal: Molecules (Basel, Switzerland) 2018;23(2):463

PMID: 29461498

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Abstract

This study was performed to evaluate the metabolite recovery from different extraction methods applied to aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min at 100 °C gave the most phenolics-enriched extract, reducing extraction time without degradation effects on bioactives. Sixteen compounds were identified in this extract, 11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis and determination of chlorophylls and carotenoids implemented the knowledge of the chemical profile of this plant.

Address: Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1, Route d'Aïn El Bey, 25000 Constantine, Algérie. [email protected].; Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1, Route d'Aïn El Bey, 25000 Constantine, Algérie. [email protected].; Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1, Route d'Aïn El Bey, 25000 Constantine, Algérie. [email protected].; Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1, Route d'Aïn El Bey, 25000 Constantine, Algérie. [email protected].; Department of Pharmacy, University "G. d'Annunzio" of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. [email protected].; Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, P.le Aldo Moro 5, 00185 Rome, Italy. [email protected].; Dipartimento di Medicina Sperimentale, Sapienza Università di Roma, P.le Aldo Moro 5, 00185 Rome, Italy. [email protected].; Department of Pharmacy, University "G. d'Annunzio" of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. [email protected].; Department of Pharmacy, University "G. d'Annunzio" of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. [email protected].; Facoltà di Bioscienze e tecnologie agro-alimentari e ambientali, Università di Teramo, Via Renato Balzarini 1, 64100 Teramo, Italy. [email protected].; Department of Pharmacy, University "G. d'Annunzio" of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. [email protected].

DOI: 10.3390/molecules23020463

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MeSH Terms: Carotenoids, Flavonoids, Phenols, Phytochemicals, Plant Components, Aerial, Plant Extracts, Thymus Plant