Colorimetric detection of 1,5-anhydroglucitol based on graphene quantum dots and enzyme-catalyzed reaction.

Zhide Zhou, Le Zhao, Zhihong Wang, Wen Xue, Yunxiao Wang, Yong Huang, Jintao Liang, Jiejing Chen, Guiyin Li

Journal: International journal of biological macromolecules 2018;112():1217-1224

PMID: 29466711

Full Text on Elsevier Science View on PubMed

Abstract

Early diagnosis of diabetes yields significant clinical benefits. The serum level of 1,5‑anhydroglucitol (1,5‑AG) has been a new biochemical marker for postprandial hyperglycemia. In this study, a simple colorimetric method for 1,5‑AG detection has been designed based on highly efficient peroxidase mimetic activity of GQDs and enzyme-catalyzed reaction. By the catalytic action of pyranose oxidase (PROD), the 1,5‑AG was oxidized to 1,5‑anhydrofuctose and HO. The GQDs in the presence of HO exhibited highly efficient catalytic activity toward the oxidation of 3, 3', 5, 5'‑tetramethylbenzidine (TMB) to a blue colored product. The influence of relevant experimental variables was optimized. A linear relationship of optical signal with the concentration of 1,5‑AG in the range of 20.0-100.0μg/mL with the regression correlation coefficient of 0.9985 was obtained which could be monitored by colorimetry detection. The limit of detection (LOD) for 1,5‑AG detection was approximately 0.144μg/mL. All in all, the proposed 1,5‑AG detection system based on GQDs and PROD-catalyzed reaction showed better performances with simple operation, low-cost, higher selectivity.

Address: School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.; Guangxi Key Laboratory of Metabolic Disease Research, Guilin 181st Hospital, Guilin, Guangxi 541014, China.; School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China.; School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China. Electronic address: [email protected].; Guangxi Key Laboratory of Metabolic Disease Research, Guilin 181st Hospital, Guilin, Guangxi 541014, China. Electronic address: [email protected].; School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China; National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning, Guangxi 530021, China. Electronic address: [email protected].

DOI: 10.1016/j.ijbiomac.2018.02.093

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MeSH Terms: Biocatalysis, Colorimetry, Deoxyglucose, Graphite, Humans, Hydrogen Peroxide, Oxidation-Reduction, Peroxidase, Quantum Dots, Solutions, Spectrophotometry, Ultraviolet