Alteromonas oceani sp. nov., isolated from deep-sea sediment of a hydrothermal field.

A novel rod-shaped, Gram-stain-negative, aerobic bacterium, designated S35, was isolated from deep-sea sediment collected from the Pacmanus hydrothermal field, Manus Basin, Papua New Guinea. Strain S35 grew optimally at 28 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain S35 shared 97.38-98.55% similarity with the type strains of Alteromonas lipolytica, Alteromonas mediterranea and Aestuariibacterhalophilus. Phylogenetic analysis showed that strain S35 belonged to the genus Alteromonas. The strain contained ubiquinone-8 as the predominant respiratory lipoquinone. Summed feature 3 (C16 : 1ω7c and/or C16 : 1ω7c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 were the major fatty acids. The DNA G+C content of strain S35 was 51.3 mol%. These results indicated that strain S35 represents a novel species of the genus Alteromonas, for which the name Alteromonas oceani sp. nov. (type strain S35=KCTC 52449=CGMCC 1.16029) is proposed.