Anticarcinogenic activities of sulforaphane are influenced by Nerve Growth Factor in human melanoma A375 cells.

Paola Arcidiacono, Anna Maria Stabile, Francesco Ragonese, Alessandra Pistilli, Stefano Calvieri, Ugo Bottoni, Andrea Crisanti, Roberta Spaccapelo, Mario Rende

Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018;113():154-161

PMID: 29407470

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Abstract

Melanoma is a severe form of cancer, resistant to conventional therapies. According to in vitro studies, sulforaphane, a dietary component, has been considered a promising antineoplastic candidate. The present study analyzes the in vitro biological effects of sulforaphane in A375 melanoma cell line with or without the addition of Nerve Growth Factor. For the first time, our results show that a supplementation of Nerve Growth Factor partially reverses the sulforaphane-induced: i) inhibition of cell migration, ii) pro apoptotic changes in cell cycle and iii) modulation of active caspase-3. Furthermore, we report the sulforaphane-induced modulation in the expression of Nerve Growth Factor receptors TrKA and p75NTR, shifting their ratio from pro survival to pro apoptotic. In conclusion, the present study evidences that in vivo the antineoplastic effects of sulforaphane may be reduced by the contemporaneous presence of other biological elements such as Nerve Growth Factor and it contributes to a better definition of the real in vivo potentiality of sulforaphane as antineoplastic candidate.

Address: Department of Experimental Medicine, School of Medicine, University of Perugia, P. le Lucio Severi 1 Sant'Andrea delle Fratte, 06132, Perugia, Italy.; Department of Surgery and Biomedical Sciences, Sect. of Human Anatomy, Clinical and Forensic, School of Medicine, University of Perugia, P. le Lucio Severi 1 Sant'Andrea delle Fratte, 06132, Perugia, Italy.; Dermatology Clinic, Department of Internal Medicine and Medical Specialties, University of Rome, Italy.; University Magna Grecia, Catanzaro, Italy.; Department of Life Science, Imperial College London, London UK.; Department of Experimental Medicine, School of Medicine, University of Perugia, P. le Lucio Severi 1 Sant'Andrea delle Fratte, 06132, Perugia, Italy. Electronic address: [email protected].; Department of Surgery and Biomedical Sciences, Sect. of Human Anatomy, Clinical and Forensic, School of Medicine, University of Perugia, P. le Lucio Severi 1 Sant'Andrea delle Fratte, 06132, Perugia, Italy. Electronic address: [email protected].

DOI: 10.1016/j.fct.2018.01.051