Protocol of Phytophthora capsici Transformation Using the CRISPR-Cas9 System.

Zhiwen Wang, Brett M Tyler, Xili Liu

Journal: Methods in molecular biology (Clifton, N.J.) 2019;1848():265-274

PMID: 30182240

Abstract

["Phytophthora capsici is an important plant pathogen, which causes significant economic losses on multiple vegetable crops worldwide. It is an ideal model pathogen to study the role of important genes, plant-pathogen interactions, and fungicide resistance mechanisms etc. due to its wide range of hosts and genetic diversity. A more efficient gene editing tool is required to do these studies. Here, we describe a detailed experimental procedure using the CRISPR-Cas9 system to edit genes of interest in P. capsici, which has been proven to be an accurate and efficient gene editing method in P. capsici."]

Address: Department of Plant Pathology, China Agricultural University, Beijing, China.; Department of Botany and Plant Pathology, Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR, USA.; Department of Plant Pathology, China Agricultural University, Beijing, China. [email protected].

DOI: 10.1007/978-1-4939-8724-5_17

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https://doi.org/10.1007/978-1-4939-8724-5_17

MeSH Terms: CRISPR-Cas Systems, Gene Editing, Gene Order, Genetic Vectors, Homologous Recombination, Mutation, Phytophthora, Protoplasts, RNA, Guide, CRISPR-Cas Systems, Transfection, Transformation, Genetic