Interaction of sucralose with whey protein: Experimental and molecular modeling studies.

Hongmei Zhang, Shixin Sun, Yanqing Wang, Jian Cao

Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2018;187():92-97

PMID: 28666158

Full Text on Elsevier Science View on PubMed

Abstract

The objective of this research was to study the interactions of sucralose with whey protein isolate (WPI) by using the three-dimensional fluorescence spectroscopy, circular dichroism spectroscopy and molecular modeling. The results showed that the peptide strands structure of WPI had been changed by sucralose. Sucralose binding induced the secondary structural changes and increased content of aperiodic structure of WPI. Sucralose decreased the thermal stability of WPI and acted as a structure destabilizer during the thermal unfolding process of protein. In addition, the existence of sucralose decreased the reversibility of the unfolding of WPI. Nonetheless, sucralose-WPI complex was less stable than protein alone. The molecular modeling result showed that van der Waals and hydrogen bonding interactions contribute to the complexation free binding energy. There are more than one possible binding sites of WPI with sucralose by surface binding mode.

Address: Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, China; Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng City, CN 224002, China.; Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, China; Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng City, CN 224002, China. Electronic address: [email protected].; Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province 224002, China; Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng City, CN 224002, China. Electronic address: [email protected].

DOI: 10.1016/j.saa.2017.06.030

Link outs

Free resources

Other Literature Sources:

https://scite.ai/reports/28666158

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S1386...

MeSH Terms: Models, Molecular, Spectrum Analysis, Sucrose, Whey Proteins