Effect of resveratrol on platelet aggregation by fibrinogen protection.

Claudia Bonechi, Stefania Lamponi, Alessandro Donati, Gabriella Tamasi, Marco Consumi, Gemma Leone, Claudio Rossi, Agnese Magnani

Journal: Biophysical chemistry 2017;222():41-48

PMID: 28095333

Full Text on Elsevier Science View on PubMed

Abstract

The effect of resveratrol (RSV) in inhibiting platelet adhesion and aggregation, as well as fibrinogen (FBG) conformational changes promoted by epinephrine (EP), were studied, by using complementary experimental techniques. NMR and IR spectroscopies were used to investigate possible protective effects by RSV towards FBG, in presence of EP. The protective effect of RSV towards FBG was highlighted by spin nuclear relaxation experiments that were interpreted for determining the thermodynamic equilibrium constants of FBG-EP interaction, and by infrared measurements, that showed EP-induced conformational changes of FBG. The ability of RSV in inhibiting platelet adhesion and aggregation promoted by EP was evaluated by scanning electron microscopy (SEM), measuring the platelet adhesion and aggregation degree, in comparison to data obtained for platelet aggregation in platelet rich plasma (PRP). The experimental combined approach pointed out that RSV is able to protect both FBG and platelets from the denaturant and aggregating action of EP at stress level concentration.

Address: Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; CSGI-Research Center for Colloids and Nanoscience, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy. Electronic address: [email protected].; Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; INSTM-National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti 9, 50121 Firenze, Italy.; Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; CSGI-Research Center for Colloids and Nanoscience, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy.

DOI: 10.1016/j.bpc.2016.12.004

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MeSH Terms: Epinephrine, Fibrinogen, Humans, Microscopy, Electrochemical, Scanning, Platelet Aggregation, Protective Agents, Protein Conformation, Resveratrol, Spectrum Analysis, Stilbenes