Platelet-released growth factors induce psoriasin in keratinocytes: Implications for the cutaneous barrier.

Andreas Bayer, Justus Lammel, Sebastian Lippross, Tim Klüter, Peter Behrendt, Mersedeh Tohidnezhad, Thomas Pufe, Jochen Cremer, Holger Jahr, Franziska Rademacher, Regine Gläser, Jürgen Harder

Journal: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2018;213():25-32

PMID: 28552640

Full Text on Elsevier Science View on PubMed

Abstract

Millions of patients around the world suffer minor or major extremity amputation due to progressive wound healing complications of chronic or infected wounds, the therapy of which remains a challenge. One emerging therapeutic option for the treatment of these complicated wounds is the local application of an autologous thrombocytes concentrate lysate (e.g. platelet-released growth factors ((PRGF)) or Vivostat PRF) that contains a multitude of chemokines, cytokines and growth factors and is therefore supposed to stimulate the complex wound healing process. Although PRGF and Vivostat PRF are already used successfully to support healing of chronic, hard-to-heal and infected wounds the underlying molecular mechanisms are not well understood. Psoriasin, also termed S100A7, is a multifunctional antimicrobial protein expressed in keratinocytes and is involved in various processes such as wound-healing, angiogenesis, innate immunity and immune-modulation. In this study, we investigated the influence of PRGF on psoriasin expression in human primary keratinocytes in vitro and the influence of Vivostat PRF on psoriasin expression in experimentally generated skin wounds in vivo. PRGF treatment of primary keratinocytes caused a significant concentration- and time-dependent increase of psoriasin gene and protein expression in vitro that were partially mediated by the epidermal growth factor receptor (EGFR) and the interleukin-6 receptor (IL-6R). In accordance with these cell culture data, Vivostat PRF induced a significant psoriasin gene and protein expression when applied to artificially generated skin wounds in vivo. The observed psoriasin induction in keratinocytes may contribute to the wound healing-promoting effects of therapeutically used thrombocyte concentrate lysates.

Address: Department of Heart- and Vascular Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller Straße 3, Haus 26, 24105 Kiel, Germany. Electronic address: [email protected].; Department of Dermatology, University Hospital of Schleswig-Holstein, Campus Kiel, Schittenhelmstr. 7, 24105 Kiel, Germany.; Department of Traumatology, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller Straße 3, Haus 18, 24105 Kiel, Germany.; Institute of Anatomy and Cell Biology, RWTH University of Aachen, Wendlingweg 2, 52072 Aachen, Germany.; Department of Heart- and Vascular Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller Straße 3, Haus 26, 24105 Kiel, Germany.; Department of Orthopedics, Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany.

DOI: 10.1016/j.aanat.2017.04.002