Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization.

Nenad B Milosavić, Radivoje M Prodanović, Dušan Velićković, Aleksandra Dimitrijević

Journal: Methods in molecular biology (Clifton, N.J.) 2018;1504():139-147

PMID: 27770419

Abstract

One of the most used procedures for enzyme stabilization is immobilization. Although immobilization on solid supports has been pursued since the 1950s, there are no general rules for selecting the best support for a giving application. A macroporous copolymer of ethylene glycol dimethacrylate and glycidyl methacrylate (poly (GMA-co-EGDMA)) is a carrier consisting of macroporous beads for immobilizing enzymes of industrial potential for the production of fine chemicals and pharmaceuticals.

Address: Division of Experimental Therapeutics, Department of Medicine, Columbia University, 630 West 168th Street, P&S 8-436, New York, NY, 10032, USA. [email protected].; Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 16, Belgrade, 11000, Serbia.

DOI: 10.1007/978-1-4939-6499-4_11

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MeSH Terms: Aspergillus niger, Cross-Linking Reagents, Enzyme Stability, Enzymes, Immobilized, Ethylene Glycols, Ethylene Oxide, Glucan 1,4-alpha-Glucosidase, Glutaral, Methacrylates, Oxidation-Reduction, Porosity