Assessment of cell death mechanisms triggered by Lu-anti-CD20 in lymphoma cells.

E Azorín-Vega, E Rojas-Calderón, B Martínez-Ventura, J Ramos-Bernal, L Serrano-Espinoza, N Jiménez-Mancilla, D Ordaz-Rosado, G Ferro-Flores

Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine 2018;138():73-77

PMID: 28434643

Full Text on Elsevier Science View on PubMed

Abstract

The aim of this research was to evaluate the cell cycle redistribution and activation of early and late apoptotic pathways in lymphoma cells after treatment with Lu-anti-CD20. Experimental and computer models were used to calculate the radiation absorbed dose to cancer cell nuclei. The computer model (Monte Carlo, PENELOPE) consisted of twenty spheres representing cells with an inner sphere (cell nucleus) embedded in culture media. Radiation emissions of the radiopharmaceutical located in cell membranes and in culture media were considered for nuclei dose calculations. Flow cytometric analyses demonstrated that doses as low as 4.8Gy are enough to induce cell cycle arrest and activate late apoptotic pathways.

Address: Gerencia de Aplicaciones Nucleares en la Salud, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México 52750, Mexico. Electronic address: [email protected].; Gerencia de Ciencias Ambientales, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México 52750, Mexico.; Gerencia de Aplicaciones Nucleares en la Salud, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México 52750, Mexico.; CONACyT, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México 52750, Mexico.; Departamento de Biología de la Reproducción, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de México 14080, Mexico.

DOI: 10.1016/j.apradiso.2017.04.016

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MeSH Terms: Apoptosis, Cell Line, Tumor, Dose-Response Relationship, Radiation, Flow Cytometry, G2 Phase Cell Cycle Checkpoints, Humans, Immunoconjugates, Lutetium, Lymphoma, Radioimmunotherapy, Radioisotopes, Rituximab