The adaptors Grb10 and Grb14 are calmodulin-binding proteins.

Irene García-Palmero, Noemí Pompas-Veganzones, Eduardo Villalobo, Sophie Gioria, Jacques Haiech, Antonio Villalobo

Journal: FEBS letters 2017;591(8):1176-1186

PMID: 28295264

Abstract

We identified the Grb7 family members, Grb10 and Grb14, as Ca -dependent CaM-binding proteins using Ca -dependent CaM-affinity chromatography as we previously did with Grb7. The potential CaM-binding sites were identified and experimentally tested using fluorescent-labeled peptides corresponding to these sites. The apparent affinity constant of these peptides for CaM, and the minimum number of calcium ions bound to CaM that are required for effective binding to these peptides were also determined. We prepared deletion mutants of the three adaptor proteins lacking the identified sites and determined that they lost or strongly diminished their CaM-binding capacity following the sequence Grb7 > > Grb14 > Grb10. More than one CaM-binding site and/or accessory CaM-binding sites appear to exist in Grb10 and Grb14, as compared to a single one present in Grb7.

Address: Department of Cancer Biology, Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Spain.; Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Spain.; Plate-forme de Chimie Biologique Intégrative de Strasbourg (PCBIS), UMS 3286 CNRS-Université de Strasbourg, France.; Laboratoire d'Excellence Medalis, Université de Strasbourg, CNRS, LIT UMR 7200, France.

DOI: 10.1002/1873-3468.12623