Lycium barbarum L. Polysaccharide (LBP) Reduces Glucose Uptake via Down-Regulation of SGLT-1 in Caco2 Cell.

Huizhen Cai, Xiaohui Yang, Qian Cai, Binbin Ren, Hongyan Qiu, Zhiqing Yao

Journal: Molecules (Basel, Switzerland) 2017;22(2):341

PMID: 28241438

Abstract

L. polysaccharide (LBP) is prepared from L. (), which is a traditional Chinese medicine. LPB has been shown to have hypoglycemic effects. In order to gain some mechanistic insights on the hypoglycemic effects of LBP, we investigated the uptake of LBP and its effect on glucose absorption in the human intestinal epithelial cell line Caco2 cell. The uptake of LBP through Caco2 cell monolayer was time-dependent and was inhibited by phloridzin, a competitive inhibitor of SGLT-1. LPB decreased the absorption of glucose in Caco2 cell, and down-regulated the expression of SGLT-1. These results suggest that LBP might be transported across the human intestinal epithelium through SGLT-1 and it inhibits glucose uptake via down-regulating SGLT-1.

Address: Department of Nutrition and Food Hygiene, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. [email protected].; Department of Sanitary Chemistry, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. [email protected].; Department of Experimental Center, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. [email protected].; Department of Experimental Center, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. [email protected].; Department of Epidemiology and Health Statistics, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. [email protected].; Department of Nutrition and Food Hygiene, School of Public Health, Ningxia Medicine University, Yinchuan 750004, Ningxia, China. [email protected].

DOI: 10.3390/molecules22020341

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Full Text Sources:

Medical:

https://medlineplus.gov/diabetesmedicines.html

Other Literature Sources:

https://scite.ai/reports/28241438

Miscellaneous:

https://assays.cancer.gov/non-CPTAC-1140

MeSH Terms: Caco-2 Cells, Carbohydrate Metabolism, Down-Regulation, Drugs, Chinese Herbal, Glucose, Glucose Transporter Type 2, Humans, Hypoglycemic Agents, Intestinal Absorption, Lycium, RNA, Messenger, Sodium-Glucose Transporter 1