Comparative transcriptomic analysis reveals phenol tolerance mechanism of evolved Chlorella strain.

Lin Zhou, Dujia Cheng, Liang Wang, Juan Gao, Quanyu Zhao, Wei Wei, Yuhan Sun

Journal: Bioresource technology 2017;227():266-272

PMID: 28040647

Full Text on Elsevier Science View on PubMed

Abstract

The growth of microalgae is inhibited by high concentration phenol due to reactive oxygen species. An evolved strain tolerated to 500mg/L phenol, Chlorella sp. L5, was obtained in previous study. In this study, comparative transcriptomic analysis was performed for Chlorella sp. L5 and its original strain (Chlorella sp. L3). The tolerance mechanism of Chlorella sp. L5 for high concentration phenol was explored on genome scale. It was identified that the up-regulations of the related genes according to antioxidant enzymes (SOD, APX, CAT and GR) and carotenoids (astaxanthin, lutein and lycopene) biosynthesis had critical roles to tolerate high concentration phenol. In addition, most of genes of PS I, PS II, photosynthetic electron transport chain and starch biosynthesis were also up-regulated. It was consistent to the experimental results of total carbohydrate contents of Chlorella sp. L3 and Chlorella sp. L5 under 0mg/L and 500mg/L phenol.

Address: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; School of Life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, China.; Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China; ShanghaiTech University, 100 Haike Road, Shanghai 201210, China.; Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China.; School of Life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, China.; Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 100 Haike Road, Shanghai 201210, China. Electronic address: [email protected].; Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 100 Haike Road, Shanghai 201210, China.

DOI: 10.1016/j.biortech.2016.12.059

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MeSH Terms: Chlorella, Drug Tolerance, Phenol, Transcriptome