Determining Diguanylate Cyclase Activity (Radioactive Assay).

Barbara I Kazmierczak

Journal: Methods in molecular biology (Clifton, N.J.) 2018;1657():285-289

PMID: 28889302

Abstract

Bioinformatics approaches can identify sequence motifs associated with diguanylate cyclases (DGCs), but experimental demonstration of DGC enzymatic activity is often desired. This protocol describes a sensitive radioactive assay for DGC activity in which substrate and product are quickly and easily separated by thin-layer chromatography.

Address: Department of Medicine, Yale University, 333 Cedar St., New Haven, CT, 06520-8022, USA. [email protected].; Department of Microbial Pathogenesis, Yale University, 333 Cedar St., New Haven, CT, 06520-8022, USA. [email protected].

DOI: 10.1007/978-1-4939-7240-1_22

Link outs

Free resources

Other Literature Sources:

https://scite.ai/reports/28889302

Subscription / membership required

Full Text Sources:

https://doi.org/10.1007/978-1-4939-7240-1_22

MeSH Terms: Chromatography, Thin Layer, Enzyme Activation, Enzyme Assays, Escherichia coli Proteins, Phosphorus Radioisotopes, Phosphorus-Oxygen Lyases