The Effect of Post-Resistance Exercise Amino Acids on Plasma MCP-1 and CCR2 Expression.

Adam J Wells, Jay R Hoffman, Adam R Jajtner, Alyssa N Varanoske, David D Church, Adam M Gonzalez, Jeremy R Townsend, Carleigh H Boone, Kayla M Baker, Kyle S Beyer, Gerald T Mangine, Leonardo P Oliveira, David H Fukuda, Jeffrey R Stout

Journal: Nutrients 2017;8(7):409

PMID: 27384580

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Plain Language Summary

Background: Innate immune response is generally considered to have an important role in tissue remodeling of the damaged localised skeletal muscle as a result of intense resistance exercise. Monocytes are leukocytes that also influence our adaptive immune system. Aim & Objective: The purpose of this study was to examine the changes in the markers of monocytes recruitment after a bout of high resistance exercise and ingestion of amino acid supplement. Method: Ten resistant trained men between the ages of 18 and 35 were recruited in this randomised crossover design study. Blood sample were taken at various time to measure and assess the plasma concentrations of monocyte chemoattractant protein 1 (MCP-1), myoglobin, cortisol and insulin concentrations; and expressions of C-C chemokine receptor-2 (CCR2), and macrophage-1 antigen (CD11b) on classical monocytes. Result & conclusion: No significant differences were noted for the markers myoglobin, insulin or cortisol in the treatment group in this study. Ingestion of supplement increased the CCR2 expression on CD14+ monocytes at 1H, 2H and 5H(P’s ≤ 0.001) after the exercise, but did not appear to have any influence on CD11b expression on CD14++CD16- ́ monocytes. To conclude it seems that amino acids are important regulator of immune response but more research is needed.

Abstract

The recruitment and infiltration of classical monocytes into damaged muscle is critical for optimal tissue remodeling. This study examined the effects of an amino acid supplement on classical monocyte recruitment following an acute bout of lower body resistance exercise. Ten resistance-trained men (24.7 ± 3.4 years; 90.1 ± 11.3 kg; 176.0 ± 4.9 cm) ingested supplement (SUPP) or placebo (PL) immediately post-exercise in a randomized, cross-over design. Blood samples were obtained at baseline (BL), immediately (IP), 30-min (30P), 1-h (1H), 2-h (2H), and 5-h (5H) post-exercise to assess plasma concentrations of monocyte chemoattractant protein 1 (MCP-1), myoglobin, cortisol and insulin concentrations; and expressions of C-C chemokine receptor-2 (CCR2), and macrophage-1 antigen (CD11b) on classical monocytes. Magnitude-based inferences were used to provide inferences on the true effects of SUPP compared to PL. Changes in myoglobin, cortisol, and insulin concentrations were similar between treatments. Compared to PL, plasma MCP-1 was "very likely greater" (98.1% likelihood effect) in SUPP at 2H. CCR2 expression was "likely greater" at IP (84.9% likelihood effect), "likely greater" at 1H (87.7% likelihood effect), "very likely greater" at 2H (97.0% likelihood effect), and "likely greater" at 5H (90.1% likelihood effect) in SUPP, compared to PL. Ingestion of SUPP did not influence CD11b expression. Ingestion of an amino acid supplement immediately post-exercise appears to help maintain plasma MCP-1 concentrations and augment CCR2 expression in resistance trained men.

Address: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Department of Health Professions, Hofstra University, Hempstead, NY 11549, USA. [email protected].; Department of Kinesiology, Lipscomb University, Nashville, TN 37204, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Department of Exercise Science and Sport Management, Kennesaw State University, Kennesaw, GA 30144, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].; Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. [email protected].

DOI: 10.3390/nu8070409

Clinical Imbalances

Immune and inflammation Structural Integrity and Musculoskeletal

Laboratory Testing

Blood Urine

Modifiable Lifestyle Factors

Diet Exercise and movement

Nutrition Evidence Keywords

Innateimmunity Resistanceexercise Aminoacidsupplementation

Bioactive Substances

Insulin Cortisol Aminoacid

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Other Literature Sources:

https://scite.ai/reports/27384580

Miscellaneous:

https://assays.cancer.gov/CPTAC-327

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-ITGAM-1

MeSH Terms: Administration, Oral, Adult, Amino Acids, Biomarkers, Body Mass Index, Body Weight, CD11b Antigen, Chemokine CCL2, Cross-Over Studies, Diet, Dietary Supplements, Humans, Hydrocortisone, Insulin, Lactic Acid, Male, Monocytes, Myoglobin, Receptors, CCR2, Resistance Training, Young Adult