Proteomic Analysis of Human Brown Adipose Tissue Reveals Utilization of Coupled and Uncoupled Energy Expenditure Pathways.

Sebastian Müller, Miroslav Balaz, Patrik Stefanicka, Lukas Varga, Ez-Zoubir Amri, Jozef Ukropec, Bernd Wollscheid, Christian Wolfrum

Journal: Scientific reports 2018;6():30030

PMID: 27418403

Free Full Text on Nature Publishing Group View on PubMed

Abstract

Human brown adipose tissue (BAT) has become an attractive target to combat the current epidemical spread of obesity and its associated co-morbidities. Currently, information on its functional role is primarily derived from rodent studies. Here, we present the first comparative proteotype analysis of primary human brown adipose tissue versus adjacent white adipose tissue, which reveals significant quantitative differences in protein abundances and in turn differential functional capabilities. The majority of the 318 proteins with increased abundance in BAT are associated with mitochondrial metabolism and confirm the increased oxidative capacity. In addition to uncoupling protein 1 (UCP1), the main functional effector for uncoupled respiration, we also detected the mitochondrial creatine kinases (CKMT1A/B, CKMT2), as effective modulators of ATP synthase coupled respiration, to be exclusively expressed in BAT. The abundant expression and utilization of both energy expenditure pathways in parallel highlights the complex functional involvement of BAT in human physiology.

Address: Institute of Food, Nutrition and Health, ETH Zurich, Schwerzenbach, Switzerland.; Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.; Life Science Zurich Graduate School, Molecular Life Sciences Program, Zurich, Switzerland.; Institute of Food, Nutrition and Health, ETH Zurich, Schwerzenbach, Switzerland.; Department of Otorhinolaryngology - Head and Neck Surgery, Faculty of Medicine and University Hospital, Comenius University, Bratislava, Slovakia.; Department of Otorhinolaryngology - Head and Neck Surgery, Faculty of Medicine and University Hospital, Comenius University, Bratislava, Slovakia.; Institute of Experimental Endocrinology, Biomedical Research Center at the Slovak Academy of Sciences, Bratislava, Slovakia.; UMR 7277, Centre National de la Recherche Scientifique, U1091, Institut National de la Santé et de la Recherche Médicale, Institute of Biology Valrose, University Nice Sophia Antipolis, Nice, France.; Institute of Experimental Endocrinology, Biomedical Research Center at the Slovak Academy of Sciences, Bratislava, Slovakia.; Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.

DOI: 10.1038/srep30030

Link outs

Free resources

Full Text Sources:

Other Literature Sources:

https://scite.ai/reports/27418403

Molecular Biology Databases:

https://data.niaid.nih.gov/resources?id=pxd003843

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-OTUB1-1

MeSH Terms: Adipose Tissue, Brown, Adipose Tissue, White, Biomarkers, Cell Line, Creatine Kinase, Creatine Kinase, Mitochondrial Form, Energy Metabolism, Gene Expression Profiling, Humans, Mitochondria, Proteome, Uncoupling Protein 1